4D6V

Crystal structure of signal transducing protein

Summary for 4D6V

• Entry DOI: 10.2210/pdb4d6v/pdb
• Descriptor: G PROTEIN BETA SUBUNIT GIB2 (2 entities in total)
• Functional Keywords: signaling protein, scaffold protein, ribosome binding
• Biological source: CRYPTOCOCCUS NEOFORMANS VAR. GRUBII H99
• Total number of polymer chains: 1
• Total formula weight: 34520.80

Authors

Ero, R.,Dimitrova, V.T.,Chen, Y.,Bu, W.,Feng, S.,Liu, T.,Wang, P.,Xue, C.,Tan, S.M.,Gao, Y.G. (deposition date: 2014-11-17, release date: 2015-03-18, Last modification date: 2023-12-20)

Primary citation

Ero, R.,Dimitrova, V.T.,Chen, Y.,Bu, W.,Feng, S.,Liu, T.,Wang, P.,Xue, C.,Tan, S.M.,Gao, Y.G.
Crystal Structure of Gib2, a Signal-Transducing Protein Scaffold Associated with Ribosomes in Cryptococcus Neoformans.
Sci.Rep., 5:8688-, 2015

PubMed Abstract: The atypical Gβ-like/RACK1 Gib2 protein promotes cAMP signalling that plays a central role in regulating the virulence of Cryptococcus neoformans. Gib2 contains a seven-bladed β transducin structure and is emerging as a scaffold protein interconnecting signalling pathways through interactions with various protein partners. Here, we present the crystal structure of Gib2 at a 2.2-Å resolution. The structure allows us to analyse the association between Gib2 and the ribosome, as well as to identify the Gib2 amino acid residues involved in ribosome binding. Our studies not only suggest that Gib2 has a role in protein translation but also present Gib2 as a physical link at the crossroads of various regulatory pathways important for the growth and virulence of C. neoformans.

PubMed: 25732347
DOI: 10.1038/SREP08688

Experimental method

X-RAY DIFFRACTION (2.2 Å)