4D69

SOYBEAN AGGLUTININ FROM GLYCINE MAX IN COMPLEX WITH THE ANTIGEN Tn

Summary for 4D69

• Entry DOI: 10.2210/pdb4d69/pdb
• Descriptor: LECTIN, SHORT ANTIGEN PEPTIDE, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: sugar-binding protein, sugar binding protein
• Biological source: GLYCINE MAX (SOYBEAN); More
• Total number of polymer chains: 24
• Total formula weight: 346930.73

Authors

Madariaga, D.,Martinez-Saez, N.,Somovilla, V.J.,Coelho, H.,Valero-Gonzalez, J.,Castro-Lopez, J.,Asensio, J.L.,Jimenez-Barbero, J.,Busto, J.H.,Avenoza, A.,Marcelo, F.,Hurtado-Guerrero, R.,Corzana, F.,Peregrina, J.M. (deposition date: 2014-11-10, release date: 2014-11-19, Last modification date: 2024-11-13)

Primary citation

Madariaga, D.,Martinez-Saez, N.,Somovilla, V.J.,Coelho, H.,Valero-Gonzalez, J.,Castro-Lopez, J.,Asensio, J.L.,Jimenez-Barbero, J.,Busto, J.H.,Avenoza, A.,Marcelo, F.,Hurtado-Guerrero, R.,Corzana, F.,Peregrina, J.M.
Detection of Tumor-Associated Glycopeptides by Lectins: The Peptide Context Modulates Carbohydrate Recognition.
Acs Chem.Biol., 10:747-, 2015

PubMed Abstract: Tn antigen (α-O-GalNAc-Ser/Thr) is a convenient cancer biomarker that is recognized by antibodies and lectins. This work yields remarkable results for two plant lectins in terms of epitope recognition and reveals that these receptors show higher affinity for Tn antigen when it is incorporated in the Pro-Asp-Thr-Arg (PDTR) peptide region of mucin MUC1. In contrast, a significant affinity loss is observed when Tn antigen is located in the Ala-His-Gly-Val-Thr-Ser-Ala (AHGVTSA) or Ala-Pro-Gly-Ser-Thr-Ala-Pro (APGSTAP) fragments. Our data indicate that the charged residues, Arg and Asp, present in the PDTR sequence establish noteworthy fundamental interactions with the lectin surface as well as fix the conformation of the peptide backbone, favoring the presentation of the sugar moiety toward the lectin. These results may help to better understand glycopeptide-lectin interactions and may contribute to engineer new binding sites, allowing novel glycosensors for Tn antigen detection to be designed.

PubMed: 25457745
DOI: 10.1021/CB500855X

Experimental method

X-RAY DIFFRACTION (2.7 Å)