4D5U

Structure of OmpF in I2

4D5U の概要

• エントリーDOI: 10.2210/pdb4d5u/pdb
• 関連するPDBエントリー: 4JFB
• 分子名称: OUTER MEMBRANE PROTEIN F (1 entity in total)
• 機能のキーワード: structural protein, porin
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cell outer membrane ; Multi-pass membrane protein : P02931
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 222685.50

構造登録者

Chaptal, V.,Kilburg, A.,Flot, D.,Wiseman, B.,Aghajari, N.,Jault, J.M.,Falson, P. (登録日: 2014-11-07, 公開日: 2015-12-16, 最終更新日: 2023-12-20)

主引用文献

Chaptal, V.,Kilburg, A.,Flot, D.,Wiseman, B.,Aghajari, N.,Jault, J.,Falson, P.
Two Different Centered Monoclinic Crystals of the E. Coli Outer-Membrane Protein Ompf Originate from the Same Building Block.
Biochim.Biophys.Acta, 1858:326-, 2016

PubMed Abstract: Macromolecule crystal formation can be divided in two major steps: 1. the formation of a nucleus and 2. the growth of this nucleus into a full mature crystal. The latter is well described and understood, while the former remains elusive due to the difficulty to study it and is described by nucleation theories. Here we report the structure of the Escherichia coli outer membrane porin OmpF in two centered monoclinic space groups. Strikingly, the two crystals originate from the same building block, made of two trimers of OmpF interacting via their rough side. The different crystallization conditions trigger the formation of distinct arrangement of these building blocks, leading to the formation of translational non-crystallographic symmetry (tNCS) in one case, made possible by the loose lateral packing mediated by detergents. In light of nucleation theories, these results allow us to speculate that these two crystals originate from nuclei made of either clusters of building blocks, or already forming columns that later associate laterally using detergents as glue.

PubMed: 26620074
DOI: 10.1016/J.BBAMEM.2015.11.021

実験手法

X-RAY DIFFRACTION (3.5 Å)