4D3S

Imine reductase from Nocardiopsis halophila

4D3S の概要

• エントリーDOI: 10.2210/pdb4d3s/pdb
• 関連するPDBエントリー: 4D3D 4D3F
• 分子名称: IMINE REDUCTASE, octyl beta-D-glucopyranoside (3 entities in total)
• 機能のキーワード: nadph, oxidoreductase
• 由来する生物種: NOCARDIOPSIS HALOPHILA
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 246646.02

構造登録者

Man, H.,Hart, S.,Turkenburg, J.P.,Grogan, G. (登録日: 2014-10-23, 公開日: 2015-04-01, 最終更新日: 2023-12-20)

主引用文献

Man, H.,Wells, E.,Hussain, S.,Leipold, F.,Hart, S.,Turkenburg, J.P.,Turner, N.J.,Grogan, G.
Structure, Activity and Stereoselectivity of Nadph-Dependent Oxidoreductases Catalysing the S-Selective Reduction of the Imine Substrate 2-Methylpyrroline.
Chembiochem, 16:1052-, 2015

PubMed Abstract: Oxidoreductases from Streptomyces sp. GF3546 [3546-IRED], Bacillus cereus BAG3X2 (BcIRED) and Nocardiopsis halophila (NhIRED) each reduce prochiral 2-methylpyrroline (2MPN) to (S)-2-methylpyrrolidine with >95 % ee and also a number of other imine substrates with good selectivity. Structures of BcIRED and NhIRED have helped to identify conserved active site residues within this subgroup of imine reductases that have S selectivity towards 2MPN, including a tyrosine residue that has a possible role in catalysis and superimposes with an aspartate in related enzymes that display R selectivity towards the same substrate. Mutation of this tyrosine residue-Tyr169-in 3546-IRED to Phe resulted in a mutant of negligible activity. The data together provide structural evidence for the location and significance of the Tyr residue in this group of imine reductases, and permit a comparison of the active sites of enzymes that reduce 2MPN with either R or S selectivity.

PubMed: 25809902
DOI: 10.1002/CBIC.201402625

実験手法

X-RAY DIFFRACTION (2.24 Å)