4D0K

Complex of Chaetomium thermophilum PAN2 (WD40-CS1) with PAN3 (C-term)

4D0K の概要

• エントリーDOI: 10.2210/pdb4d0k/pdb
• 関連するPDBエントリー: 4CZV 4CZW 4CZX 4CZY
• 分子名称: A-SPECIFIC RIBONUCLEASE SUBUNIT PAN2, PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUNIT PAN3-LIKE PROTEIN (3 entities in total)
• 機能のキーワード: gene regulation, wd40 domain, c-terminal knob domain, deadenylation, mrna decay, pan2-pan3 complex
• 由来する生物種: CHAETOMIUM THERMOPHILUM; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 82441.18

構造登録者

Jonas, S.,Izaurralde, E.,Weichenrieder, O. (登録日: 2014-04-28, 公開日: 2014-06-04, 最終更新日: 2023-12-20)

主引用文献

Jonas, S.,Christie, M.,Peter, D.,Bhandari, D.,Loh, B.,Huntzinger, E.,Weichenrieder, O.,Izaurralde, E.
An Asymmetric Pan3 Dimer Recruits a Single Pan2 Exonuclease to Mediate Mrna Deadenylation and Decay.
Nat.Struct.Mol.Biol., 21:599-, 2014

PubMed Abstract: The PAN2-PAN3 complex functions in general and microRNA-mediated mRNA deadenylation. However, mechanistic insight into PAN2 and its complex with the asymmetric PAN3 dimer is lacking. Here, we describe crystal structures that show that Neurospora crassa PAN2 comprises two independent structural units: a C-terminal catalytic unit and an N-terminal assembly unit that engages in a bipartite interaction with PAN3 dimers. The catalytic unit contains the exonuclease domain in an intimate complex with a potentially modulatory ubiquitin-protease-like domain. The assembly unit contains a WD40 propeller connected to an adaptable linker. The propeller contacts the PAN3 C-terminal domain, whereas the linker reinforces the asymmetry of the PAN3 dimer and prevents the recruitment of a second PAN2 molecule. Functional data indicate an essential role for PAN3 in coordinating PAN2-mediated deadenylation with subsequent steps in mRNA decay, which lead to complete mRNA degradation.

PubMed: 24880343
DOI: 10.1038/NSMB.2837

実験手法

X-RAY DIFFRACTION (1.89 Å)