4CZK

C. crescentus MreB, single filament, AMPPNP, MP265 inhibitor

Summary for 4CZK

• Entry DOI: 10.2210/pdb4czk/pdb
• Related: 4CZE 4CZF 4CZG 4CZH 4CZI 4CZJ 4CZL 4CZM
• Descriptor: ROD SHAPE-DETERMINING PROTEIN MREB, MAGNESIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (5 entities in total)
• Functional Keywords: structural protein, bacterial actin, bacterial cytoskeleton
• Biological source: CAULOBACTER VIBRIOIDES
• Total number of polymer chains: 1
• Total formula weight: 37596.69

Authors

Lowe, J.,van den Ent, F. (deposition date: 2014-04-19, release date: 2014-06-11, Last modification date: 2023-12-20)

Primary citation

Van Den Ent, F.,Izore, T.,Bharat, T.A.,Johnson, C.M.,Lowe, J.
Bacterial Actin Mreb Forms Antiparallel Double Filaments.
Elife, 3:02634-, 2014

PubMed Abstract: Filaments of all actin-like proteins known to date are assembled from pairs of protofilaments that are arranged in a parallel fashion, generating polarity. In this study, we show that the prokaryotic actin homologue MreB forms pairs of protofilaments that adopt an antiparallel arrangement in vitro and in vivo. We provide an atomic view of antiparallel protofilaments of Caulobacter MreB as apparent from crystal structures. We show that a protofilament doublet is essential for MreB's function in cell shape maintenance and demonstrate by in vivo site-specific cross-linking the antiparallel orientation of MreB protofilaments in E. coli. 3D cryo-EM shows that pairs of protofilaments of Caulobacter MreB tightly bind to membranes. Crystal structures of different nucleotide and polymerisation states of Caulobacter MreB reveal conserved conformational changes accompanying antiparallel filament formation. Finally, the antimicrobial agents A22/MP265 are shown to bind close to the bound nucleotide of MreB, presumably preventing nucleotide hydrolysis and destabilising double protofilaments.DOI: http://dx.doi.org/10.7554/eLife.02634.001.

PubMed: 24843005
DOI: 10.7554/ELIFE.02634

Experimental method

X-RAY DIFFRACTION (2.602 Å)