4CY3
Crystal structure of the NSL1-WDS complex.
Summary for 4CY3
| Entry DOI | 10.2210/pdb4cy3/pdb |
| Related | 4CY1 4CY2 4CY5 |
| Descriptor | PROTEIN WILL DIE SLOWLY, CG4699, ISOFORM D, GLYCEROL, ... (4 entities in total) |
| Functional Keywords | transcription, epigenetic regulator, histone acetylation, chromatin |
| Biological source | DROSOPHILA MELANOGASTER (FRUIT FLY) More |
| Total number of polymer chains | 2 |
| Total formula weight | 36533.39 |
| Authors | Dias, J.,Brettschneider, J.,Cusack, S.,Kadlec, J. (deposition date: 2014-04-10, release date: 2014-05-14, Last modification date: 2023-12-20) |
| Primary citation | Dias, J.,Van Nguyen, N.,Georgiev, P.,Gaub, A.,Brettschneider, J.,Cusack, S.,Kadlec, J.,Akhtar, A. Structural Analysis of the Kansl1/Wdr5/Kansl2 Complex Reveals that Wdr5 is Required for Efficient Assembly and Chromatin Targeting of the Nsl Complex. Genes Dev., 28:929-, 2014 Cited by PubMed Abstract: The subunits of the nonspecific lethal (NSL) complex, which include the histone acetyltransferase MOF (males absent on the first), play important roles in various cellular functions, including transcription regulation and stem cell identity maintenance and reprogramming, and are frequently misregulated in disease. Here, we provide the first biochemical and structural insights into the molecular architecture of this large multiprotein assembly. We identified several direct interactions within the complex and show that KANSL1 acts as a scaffold protein interacting with four other subunits, including WDR5, which in turn binds KANSL2. Structural analysis of the KANSL1/WDR5/KANSL2 subcomplex reveals how WDR5 is recruited into the NSL complex via conserved linear motifs of KANSL1 and KANSL2. Using structure-based KANSL1 mutants in transgenic flies, we show that the KANSL1-WDR5 interaction is required for proper assembly, efficient recruitment of the NSL complex to target promoters, and fly viability. Our data clearly show that the interactions of WDR5 with the MOF-containing NSL complex and MLL/COMPASS histone methyltransferase complexes are mutually exclusive. We propose that rather than being a shared subunit, WDR5 plays an important role in assembling distinct histone-modifying complexes with different epigenetic regulatory roles. PubMed: 24788516DOI: 10.1101/GAD.240200.114 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.4 Å) |
Structure validation
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