4CS5
Crystal Structure of PCNA from Litopenaeus vannamei
Summary for 4CS5
| Entry DOI | 10.2210/pdb4cs5/pdb |
| Descriptor | PROLIFERATING CELL NUCLEAR ANTIGEN (1 entity in total) |
| Functional Keywords | nuclear protein, dna binding protein |
| Biological source | LITOPENAEUS VANNAMEI (PACIFIC WHITE SHRIMP) |
| Cellular location | Nucleus (By similarity): G1E6N7 |
| Total number of polymer chains | 3 |
| Total formula weight | 86520.20 |
| Authors | Carrasco-Miranda, J.S.,Lopez-Zavala, A.A.,De-La-Mora, E.,Rudino-Pinera, E.,Brieba, L.G.,Sotelo-Mundo, R.R. (deposition date: 2014-03-04, release date: 2014-04-23, Last modification date: 2023-12-20) |
| Primary citation | Carrasco-Miranda, J.S.,Lopez-Zavala, A.A.,Arvizu-Flores, A.A.,Garcia-Orozco, K.D.,Stojanoff, V.,Rudino-Pinera, E.,Brieba, L.G.,Sotelo-Mundo, R.R. Crystal Structure of the Shrimp Proliferating Cell Nuclear Antigen: Structural Complementarity with Wssv DNA Polymerase Pip-Box. Plos One, 9:94369-, 2014 Cited by PubMed Abstract: DNA replication requires processivity factors that allow replicative DNA polymerases to extend long stretches of DNA. Some DNA viruses encode their own replicative DNA polymerase, such as the white spot syndrome virus (WSSV) that infects decapod crustaceans but still require host replication accessory factors. We have determined by X-ray diffraction the three-dimensional structure of the Pacific white leg shrimp Litopenaeus vannamei Proliferating Cell Nuclear Antigen (LvPCNA). This protein is a member of the sliding clamp family of proteins, that binds DNA replication and DNA repair proteins through a motif called PIP-box (PCNA-Interacting Protein). The crystal structure of LvPCNA was refined to a resolution of 3 Å, and allowed us to determine the trimeric protein assembly and details of the interactions between PCNA and the DNA. To address the possible interaction between LvPCNA and the viral DNA polymerase, we docked a theoretical model of a PIP-box peptide from the WSSV DNA polymerase within LvPCNA crystal structure. The theoretical model depicts a feasible model of interaction between both proteins. The crystal structure of shrimp PCNA allows us to further understand the mechanisms of DNA replication processivity factors in non-model systems. PubMed: 24728082DOI: 10.1371/JOURNAL.PONE.0094369 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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