4CS5

Crystal Structure of PCNA from Litopenaeus vannamei

4CS5 の概要

• エントリーDOI: 10.2210/pdb4cs5/pdb
• 分子名称: PROLIFERATING CELL NUCLEAR ANTIGEN (1 entity in total)
• 機能のキーワード: nuclear protein, dna binding protein
• 由来する生物種: LITOPENAEUS VANNAMEI (PACIFIC WHITE SHRIMP)
• 細胞内の位置: Nucleus (By similarity): G1E6N7
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 86520.20

構造登録者

Carrasco-Miranda, J.S.,Lopez-Zavala, A.A.,De-La-Mora, E.,Rudino-Pinera, E.,Brieba, L.G.,Sotelo-Mundo, R.R. (登録日: 2014-03-04, 公開日: 2014-04-23, 最終更新日: 2023-12-20)

主引用文献

Carrasco-Miranda, J.S.,Lopez-Zavala, A.A.,Arvizu-Flores, A.A.,Garcia-Orozco, K.D.,Stojanoff, V.,Rudino-Pinera, E.,Brieba, L.G.,Sotelo-Mundo, R.R.
Crystal Structure of the Shrimp Proliferating Cell Nuclear Antigen: Structural Complementarity with Wssv DNA Polymerase Pip-Box.
Plos One, 9:94369-, 2014

PubMed Abstract: DNA replication requires processivity factors that allow replicative DNA polymerases to extend long stretches of DNA. Some DNA viruses encode their own replicative DNA polymerase, such as the white spot syndrome virus (WSSV) that infects decapod crustaceans but still require host replication accessory factors. We have determined by X-ray diffraction the three-dimensional structure of the Pacific white leg shrimp Litopenaeus vannamei Proliferating Cell Nuclear Antigen (LvPCNA). This protein is a member of the sliding clamp family of proteins, that binds DNA replication and DNA repair proteins through a motif called PIP-box (PCNA-Interacting Protein). The crystal structure of LvPCNA was refined to a resolution of 3 Å, and allowed us to determine the trimeric protein assembly and details of the interactions between PCNA and the DNA. To address the possible interaction between LvPCNA and the viral DNA polymerase, we docked a theoretical model of a PIP-box peptide from the WSSV DNA polymerase within LvPCNA crystal structure. The theoretical model depicts a feasible model of interaction between both proteins. The crystal structure of shrimp PCNA allows us to further understand the mechanisms of DNA replication processivity factors in non-model systems.

PubMed: 24728082
DOI: 10.1371/JOURNAL.PONE.0094369

実験手法

X-RAY DIFFRACTION (3 Å)