4CS1

Crystal structure of a simple duplex kink turn, HmKt-7 with 2 Mg bound.

4CS1 の概要

• エントリーDOI: 10.2210/pdb4cs1/pdb
• 分子名称: 5'-(*GP*GP*CP*GP*AP*AP*GP*AP*AP*CP*CP*GP*GP*GP *GP*AP*GP*CP*CP)-3', MAGNESIUM ION (3 entities in total)
• 機能のキーワード: rna, kink turn, metal ion
• 由来する生物種: HALOARCULA MARISMORTUI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6282.44

構造登録者

Huang, L.,Lilley, D.M.J. (登録日: 2014-03-03, 公開日: 2014-11-19, 最終更新日: 2023-12-20)

主引用文献

Mcphee, S.A.,Huang, L.,Lilley, D.M.J.
A Critical Base Pair in K-Turns that Confers Folding Characteristics and Correlates with Biological Function.
Nat.Commun., 5:5127-, 2014

PubMed Abstract: Kink turns (k-turns) are widespread elements in RNA that mediate tertiary contacts by kinking the helical axis. We have found that the ability of k-turns to undergo ion-induced folding is conferred by a single base pair that follows the conserved A·G pairs, that is, the 3b·3n position. A Watson-Crick pair leads to an inability to fold in metal ions alone, while 3n=G or 3b=C (but not both) permits folding. Crystallographic study reveals two hydrated metal ions coordinated to O6 of G3n and G2n of Kt-7. Removal of either atom impairs Mg(2+)-induced folding in solution. While SAM-I riboswitches have 3b·3n sequences that would predispose them to ion-induced folding, U4 snRNA are strongly biased to an inability to such folding. Thus riboswitch sequences allow folding to occur independently of protein binding, while U4 should remain unfolded until bound by protein. The empirical rules deduced for k-turn folding have strong predictive value.

PubMed: 25351101
DOI: 10.1038/NCOMMS6127

実験手法

X-RAY DIFFRACTION (2 Å)