4CQH
Structure of Infrared Fluorescent Protein IFP2.0
4CQH の概要
エントリーDOI | 10.2210/pdb4cqh/pdb |
分子名称 | BACTERIOPHYTOCHROME, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid, SODIUM ION, ... (4 entities in total) |
機能のキーワード | fluorescent protein, infrared fluorescent protein |
由来する生物種 | DEINOCOCCUS RADIODURANS |
タンパク質・核酸の鎖数 | 1 |
化学式量合計 | 36600.63 |
構造登録者 | Lafaye, C.,Yu, D.,Noirclerc-Savoye, M.,Shu, X.,Royant, A. (登録日: 2014-02-17, 公開日: 2014-05-28, 最終更新日: 2024-11-06) |
主引用文献 | Yu, D.,Gustafson, W.C.,Han, C.,Lafaye, C.,Noirclerc-Savoye, M.,Ge, W.,Thayer, D.A.,Huang, H.,Kornberg, T.B.,Royant, A.,Jan, L.Y.,Jan, Y.N.,Weiss, W.A.,Shu, X. An Improved Monomeric Infrared Fluorescent Protein for Neuronal and Tumour Brain Imaging. Nat.Commun., 5:3626-, 2014 Cited by PubMed Abstract: Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monomeric versions of these proteins can be advantageous as protein tags or for sensor development. In contrast to GFP, which requires only molecular oxygen for chromophore maturation, phytochrome-derived IFPs incorporate biliverdin (BV) as the chromophore. However, BV varies in concentration in different cells and organisms. Here we engineered cells to express the haeme oxygenase responsible for BV biosynthesis and a brighter monomeric IFP mutant (IFP2.0). Together, these tools improve the imaging capabilities of IFP2.0 compared with monomeric IFP1.4 and dimeric iRFP. By targeting IFP2.0 to the plasma membrane, we demonstrate robust labelling of neuronal processes in Drosophila larvae. We also show that this strategy improves the sensitivity when imaging brain tumours in whole mice. Our work shows promise in the application of IFPs for protein labelling and in vivo imaging. PubMed: 24832154DOI: 10.1038/NCOMMS4626 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (1.14 Å) |
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