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4CQH

Structure of Infrared Fluorescent Protein IFP2.0

4CQH の概要
エントリーDOI10.2210/pdb4cqh/pdb
分子名称BACTERIOPHYTOCHROME, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid, SODIUM ION, ... (4 entities in total)
機能のキーワードfluorescent protein, infrared fluorescent protein
由来する生物種DEINOCOCCUS RADIODURANS
タンパク質・核酸の鎖数1
化学式量合計36600.63
構造登録者
Lafaye, C.,Yu, D.,Noirclerc-Savoye, M.,Shu, X.,Royant, A. (登録日: 2014-02-17, 公開日: 2014-05-28, 最終更新日: 2024-11-06)
主引用文献Yu, D.,Gustafson, W.C.,Han, C.,Lafaye, C.,Noirclerc-Savoye, M.,Ge, W.,Thayer, D.A.,Huang, H.,Kornberg, T.B.,Royant, A.,Jan, L.Y.,Jan, Y.N.,Weiss, W.A.,Shu, X.
An Improved Monomeric Infrared Fluorescent Protein for Neuronal and Tumour Brain Imaging.
Nat.Commun., 5:3626-, 2014
Cited by
PubMed Abstract: Infrared fluorescent proteins (IFPs) are ideal for in vivo imaging, and monomeric versions of these proteins can be advantageous as protein tags or for sensor development. In contrast to GFP, which requires only molecular oxygen for chromophore maturation, phytochrome-derived IFPs incorporate biliverdin (BV) as the chromophore. However, BV varies in concentration in different cells and organisms. Here we engineered cells to express the haeme oxygenase responsible for BV biosynthesis and a brighter monomeric IFP mutant (IFP2.0). Together, these tools improve the imaging capabilities of IFP2.0 compared with monomeric IFP1.4 and dimeric iRFP. By targeting IFP2.0 to the plasma membrane, we demonstrate robust labelling of neuronal processes in Drosophila larvae. We also show that this strategy improves the sensitivity when imaging brain tumours in whole mice. Our work shows promise in the application of IFPs for protein labelling and in vivo imaging.
PubMed: 24832154
DOI: 10.1038/NCOMMS4626
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (1.14 Å)
構造検証レポート
Validation report summary of 4cqh
検証レポート(詳細版)ダウンロードをダウンロード

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件を2024-11-06に公開中

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