4CP5

ndpK in complex with (Rp)-SPMPApp

4CP5 の概要

• エントリーDOI: 10.2210/pdb4cp5/pdb
• 分子名称: NUCLEOSIDE DIPHOSPHATE KINASE, CYTOSOLIC, [[(2R)-1-(6-aminopurin-9-yl)propan-2-yl]oxymethyl-sulfanyl-phosphoryl] phosphono hydrogen phosphate (3 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: DICTYOSTELIUM DISCOIDEUM
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 103190.88

構造登録者

Priet, S.,Ferron, F.,Alvarez, K.,Verron, M.,Canard, B. (登録日: 2014-01-31, 公開日: 2015-02-11, 最終更新日: 2023-12-20)

主引用文献

Priet, S.,Roux, L.,Saez-Ayala, M.,Ferron, F.,Canard, B.,Alvarez, K.
Enzymatic Synthesis of Acyclic Nucleoside Thiophosphonate Diphosphates: Effect of the Alpha-Phosphorus Configuration on HIV-1 RT Activity.
Antiviral Res., 117:122-, 2015

PubMed Abstract: The acyclic nucleosides thiophosphonates (9-[2-(thiophosphonomethoxy)ethyl]adenine (S-PMEA) and (R)-9-[2-(thiophosphonomethoxy)propyl]adenine (S-PMPA), exhibit antiviral activity against HIV-1, -2 and HBV. Their diphosphate forms S-PMEApp and S-PMPApp, synthesized as stereoisomeric mixture, are potent inhibitors of wild-type (WT) HIV-1 RT. Understanding HIV-1 RT stereoselectivity, however, awaits resolution of the diphosphate forms into defined stereoisomers. To this aim, thiophosphonate monophosphates S-PMEAp and S-PMPAp were synthesized and used in a stereocontrolled enzyme-catalyzed phosphoryl transfer reaction involving either nucleoside diphosphate kinase (NDPK) or creatine kinase (CK) to obtain thiophosphonate diphosphates as separated isomers. We then quantified substrate preference of recombinant WT HIV-1 RT toward pure stereoisomers using in vitro steady-state kinetic analyses. The crystal structure of a complex between Dictyostelium NDPK and S-PMPApp at 2.32Å allowed to determine the absolute configuration at the α-phosphorus atom in relation to the stereo-preference of studied enzymes. The RP isomer of S-PMPApp and S-PMEApp are the preferred substrate over SP for both NDPK and HIV-1 RT.

PubMed: 25766862
DOI: 10.1016/J.ANTIVIRAL.2015.03.003

実験手法

X-RAY DIFFRACTION (2.32 Å)