4CNR

Surface residue engineering of bovine carbonic anhydrase to an extreme halophilic enzyme for potential application in postcombustion CO2 capture

Summary for 4CNR

• Entry DOI: 10.2210/pdb4cnr/pdb
• Related: 4CNV 4CNW 4CNX
• Descriptor: CARBONIC ANHYDRASE 2, ZINC ION (3 entities in total)
• Functional Keywords: lyase, protein engineering, co2 capture
• Biological source: BOS TAURUS (CATTLE)
• Total number of polymer chains: 4
• Total formula weight: 117932.96

Authors

Warden, A.,Newman, J.,Peat, T.S.,Seabrook, S.,Williams, M.,Dojchinov, G.,Haritos, V. (deposition date: 2014-01-24, release date: 2015-02-04, Last modification date: 2023-12-20)

Primary citation

Warden, A.C.,Williams, M.,Peat, T.S.,Seabrook, S.A.,Newman, J.,Dojchinov, G.,Haritos, V.S.
Rational Engineering of a Mesohalophilic Carbonic Anhydrase to an Extreme Halotolerant Biocatalyst.
Nat.Commun., 6:10278-, 2015

PubMed Abstract: Enzymes expressed by highly salt-tolerant organisms show many modifications compared with salt-affected counterparts including biased amino acid and lower α-helix content, lower solvent accessibility and negative surface charge. Here, we show that halotolerance can be generated in an enzyme solely by modifying surface residues. Rational design of carbonic anhydrase II is undertaken in three stages replacing 18 residues in total, crystal structures confirm changes are confined to surface residues. Catalytic activities and thermal unfolding temperatures of the designed enzymes increase at high salt concentrations demonstrating their shift to halotolerance, whereas the opposite response is found in the wild-type enzyme. Molecular dynamics calculations reveal a key role for sodium ions in increasing halotolerant enzyme stability largely through interactions with the highly ordered first Na(+) hydration shell. For the first time, an approach to generate extreme halotolerance, a trait with broad application in industrial biocatalysis, in a wild-type enzyme is demonstrated.

PubMed: 26687908
DOI: 10.1038/NCOMMS10278

Experimental method

X-RAY DIFFRACTION (2.29 Å)