4CJD

Crystal structure of Neisseria meningitidis trimeric autotransporter and vaccine antigen NadA

4CJD の概要

• エントリーDOI: 10.2210/pdb4cjd/pdb
• 分子名称: NADA, IODIDE ION (3 entities in total)
• 機能のキーワード: cell adhesion, trimeric auto-transporter (taa), coiled-coil
• 由来する生物種: NEISSERIA MENINGITIDIS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22858.10

構造登録者

Malito, E.,Biancucci, M.,Spraggon, G.,Bottomley, M.J. (登録日: 2013-12-19, 公開日: 2014-11-26, 最終更新日: 2024-05-08)

主引用文献

Malito, E.,Biancucci, M.,Faleri, A.,Ferlenghi, I.,Scarselli, M.,Maruggi, G.,Lo Surdo, P.,Veggi, D.,Liguori, A.,Santini, L.,Bertoldi, I.,Petracca, R.,Marchi, S.,Romagnoli, G.,Cartocci, E.,Vercellino, I.,Savino, S.,Spraggon, G.,Norais, N.,Pizza, M.,Rappuoli, R.,Masignani, V.,Bottomley, M.J.
Structure of the Meningococcal Vaccine Antigen Nada and Epitope Mapping of a Bactericidal Antibody.
Proc.Natl.Acad.Sci.USA, 111:17128-, 2014

PubMed Abstract: Serogroup B Neisseria meningitidis (MenB) is a major cause of severe sepsis and invasive meningococcal disease, which is associated with 5-15% mortality and devastating long-term sequelae. Neisserial adhesin A (NadA), a trimeric autotransporter adhesin (TAA) that acts in adhesion to and invasion of host epithelial cells, is one of the three antigens discovered by genome mining that are part of the MenB vaccine that recently was approved by the European Medicines Agency. Here we present the crystal structure of NadA variant 5 at 2 Å resolution and transmission electron microscopy data for NadA variant 3 that is present in the vaccine. The two variants show similar overall topology with a novel TAA fold predominantly composed of trimeric coiled-coils with three protruding wing-like structures that create an unusual N-terminal head domain. Detailed mapping of the binding site of a bactericidal antibody by hydrogen/deuterium exchange MS shows that a protective conformational epitope is located in the head of NadA. These results provide information that is important for elucidating the biological function and vaccine efficacy of NadA.

PubMed: 25404323
DOI: 10.1073/PNAS.1419686111

実験手法

X-RAY DIFFRACTION (2.056 Å)