4CJ7

Structure of Crenactin, an archeal actin-like protein

4CJ7 の概要

• エントリーDOI: 10.2210/pdb4cj7/pdb
• 分子名称: ACTIN/ACTIN FAMILY PROTEIN, ADENOSINE-5'-DIPHOSPHATE (2 entities in total)
• 機能のキーワード: structural protein, parm, archea, filament
• 由来する生物種: PYROBACULUM CALIDIFONTIS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 97705.37

構造登録者

Izore, T.,Duman, R.E.,Kureisaite-Ciziene, D.,Lowe, J. (登録日: 2013-12-19, 公開日: 2014-01-22, 最終更新日: 2023-12-20)

主引用文献

Izore, T.,Duman, R.,Kureisaite-Ciziene, D.,Lowe, J.
Crenactin from Pyrobaculum Calidifontis is Closely Related to Actin in Structure and Forms Steep Helical Filaments.
FEBS Lett., 588:776-, 2014

PubMed Abstract: Polymerising proteins of the actin family are nearly ubiquitous. Crenactins, restricted to Crenarchaea, are more closely related to actin than bacterial MreB. Crenactins occur in gene clusters hinting at an unknown, but conserved function. We solved the crystal structure of crenactin at 3.2 Å resolution. The protein crystallises as a continuous right-handed helix with 8 subunits per complete turn, spanning 419 Å. The structure of crenactin shows several loops that are longer than in actin, but overall, crenactin is closely related to eukaryotic actin, with an RMSD of 1.6 Å. Crenactin filaments imaged by electron microscopy showed polymers with very similar helical parameters.

PubMed: 24486010
DOI: 10.1016/J.FEBSLET.2014.01.029

実験手法

X-RAY DIFFRACTION (3.2 Å)