4CIP

Spectroscopically-validated structure of ferrous cytochrome c prime from Alcaligenes xylosoxidans, reduced using ascorbate

4CIP の概要

• エントリーDOI: 10.2210/pdb4cip/pdb
• 分子名称: CYTOCHROME C', HEME C, ASCORBIC ACID, ... (5 entities in total)
• 機能のキーワード: oxidoreductase, resonance raman, validation, gas sensor, ligand discrimination, ferric, haem, heme, radiolysis
• 由来する生物種: ACHROMOBACTER XYLOSOXIDANS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14522.13

構造登録者

Kekilli, D.,Dworkowski, F.,Antonyuk, S.,Hough, M.A. (登録日: 2013-12-13, 公開日: 2014-05-21, 最終更新日: 2024-11-06)

主引用文献

Kekilli, D.,Dworkowski, F.S.,Pompidor, G.,Fuchs, M.R.,Andrew, C.R.,Antonyuk, S.,Strange, R.W.,Eady, R.R.,Hasnain, S.S.,Hough, M.A.
Fingerprinting Redox and Ligand States in Haemprotein Crystal Structures Using Resonance Raman Spectroscopy.
Acta Crystallogr.,Sect.D, 70:1289-, 2014

PubMed Abstract: It is crucial to assign the correct redox and ligand states to crystal structures of proteins with an active redox centre to gain valid functional information and prevent the misinterpretation of structures. Single-crystal spectroscopies, particularly when applied in situ at macromolecular crystallography beamlines, allow spectroscopic investigations of redox and ligand states and the identification of reaction intermediates in protein crystals during the collection of structural data. Single-crystal resonance Raman spectroscopy was carried out in combination with macromolecular crystallography on Swiss Light Source beamline X10SA using cytochrome c' from Alcaligenes xylosoxidans. This allowed the fingerprinting and validation of different redox and ligand states, identification of vibrational modes and identification of intermediates together with monitoring of radiation-induced changes. This combined approach provides a powerful tool to obtain complementary data and correctly assign the true oxidation and ligand state(s) in redox-protein crystals.

PubMed: 24816098
DOI: 10.1107/S1399004714004039

実験手法

X-RAY DIFFRACTION (1.22 Å)