4CBO

Crystal structure of Complement Factor D mutant R202A after ensemble refinement

4CBO の概要

• エントリーDOI: 10.2210/pdb4cbo/pdb
• 関連するPDBエントリー: 4CBN
• 分子名称: COMPLEMENT FACTOR D, GLYCEROL (3 entities in total)
• 機能のキーワード: hydrolase, factor d, ensemble refinement
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Secreted: P00746
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48889.57

構造登録者

Forneris, F.,Burnley, B.T.,Gros, P. (登録日: 2013-10-15, 公開日: 2013-12-18, 最終更新日: 2024-10-23)

主引用文献

Forneris, F.,Burnley, B.T.,Gros, P.
Ensemble Refinement Shows Conformational Flexibility in Crystal Structures of Human Complement Factor D
Acta Crystallogr.,Sect.D, 70:733-, 2014

PubMed Abstract: Human factor D (FD) is a self-inhibited thrombin-like serine proteinase that is critical for amplification of the complement immune response. FD is activated by its substrate through interactions outside the active site. The substrate-binding, or `exosite', region displays a well defined and rigid conformation in FD. In contrast, remarkable flexibility is observed in thrombin and related proteinases, in which Na(+) and ligand binding is implied in allosteric regulation of enzymatic activity through protein dynamics. Here, ensemble refinement (ER) of FD and thrombin crystal structures is used to evaluate structure and dynamics simultaneously. A comparison with previously published NMR data for thrombin supports the ER analysis. The R202A FD variant has enhanced activity towards artificial peptides and simultaneously displays active and inactive conformations of the active site. ER revealed pronounced disorder in the exosite loops for this FD variant, reminiscent of thrombin in the absence of the stabilizing Na(+) ion. These data indicate that FD exhibits conformational dynamics like thrombin, but unlike in thrombin a mechanism has evolved in FD that locks the unbound native state into an ordered inactive conformation via the self-inhibitory loop. Thus, ensemble refinement of X-ray crystal structures may represent an approach alternative to spectroscopy to explore protein dynamics in atomic detail.

PubMed: 24598742
DOI: 10.1107/S1399004713032549

実験手法

X-RAY DIFFRACTION (1.8 Å)