4CAT

THREE-DIMENSIONAL STRUCTURE OF CATALASE FROM PENICILLIUM VITALE AT 2.0 ANGSTROMS RESOLUTION

4CAT の概要

• エントリーDOI: 10.2210/pdb4cat/pdb
• 分子名称: CATALASE, PROTOPORPHYRIN IX CONTAINING FE (2 entities in total)
• 機能のキーワード: oxidoreductase(h2o2(a))
• 由来する生物種: Penicillium janthinellum
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 113437.46

構造登録者

Vainshtein, B.K.,Melik-Adamyan, W.R.,Barynin, V.V.,Vagin, A.A.,Grebenko, A.I. (登録日: 1983-02-24, 公開日: 1983-09-06, 最終更新日: 2023-09-27)

主引用文献

Vainshtein, B.K.,Melik-Adamyan, W.R.,Barynin, V.V.,Vagin, A.A.,Grebenko, A.I.,Borisov, V.V.,Bartels, K.S.,Fita, I.,Rossmann, M.G.
Three-dimensional structure of catalase from Penicillium vitale at 2.0 A resolution.
J.Mol.Biol., 188:49-61, 1986

PubMed Abstract: The three-dimensional structure analysis of crystalline fungal catalase from Penicillium vitale has been extended to 2.0 A resolution. The crystals belong to space group P3(1)21, with the unit cell parameters of a = b = 144.4 A and c = 133.8 A. The asymmetric unit contains half a tetrameric molecule of 222 symmetry. Each subunit is a single polypeptide chain of approximately 670 amino acid residues and binds one heme group. The amino acid sequence has been tentatively determined by computer graphics model building (using the FRODO system) and comparison with the known sequence of beef liver catalase. The atomic model has been refined by the Hendrickson & Konnert (1981) restrained least-squares program against 68,000 reflections between 5 A and 2 A resolution. The final R-factor is 0.31 after 24 refinement cycles. The secondary and tertiary structure of the catalase has been analyzed.

PubMed: 3712443
DOI: 10.1016/0022-2836(86)90479-1

実験手法

X-RAY DIFFRACTION (3 Å)