4C8E

IspF (Burkholderia cenocepacia) 2CMP complex

Summary for 4C8E

• Entry DOI: 10.2210/pdb4c8e/pdb
• Related: 4C81 4C82 4C8G 4C8I
• Descriptor: 2-C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE, ZINC ION, CYTIDINE-5'-MONOPHOSPHATE, ... (6 entities in total)
• Functional Keywords: lyase
• Biological source: BURKHOLDERIA CENOCEPACIA
• Total number of polymer chains: 3
• Total formula weight: 60267.10

Authors

O'Rourke, P.E.F.,Kalinowska-Tluscik, J.,Fyfe, P.K.,Dawson, A.,Hunter, W.N. (deposition date: 2013-09-30, release date: 2014-01-08, Last modification date: 2023-12-20)

Primary citation

O Rourke, P.E.,Kalinowska-Tluscik, J.,Fyfe, P.K.,Dawson, A.,Hunter, W.N.
Crystal Structures of Ispf from Plasmodium Falciparum and Burkholderia Cenocepacia: Comparisons Inform Antimicrobial Drug Target Assessment.
Bmc Struct.Biol., 14:1-, 2014

PubMed Abstract: 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase (IspF) catalyzes the conversion of 4-diphosphocytidyl-2C-methyl-D-erythritol-2-phosphate to 2C-methyl-D-erythritol-2,4-cyclodiphosphate and cytidine monophosphate in production of isoprenoid-precursors via the methylerythritol phosphate biosynthetic pathway. IspF is found in the protozoan Plasmodium falciparum, a parasite that causes cerebral malaria, as well as in many Gram-negative bacteria such as Burkholderia cenocepacia. IspF represents a potential target for development of broad-spectrum antimicrobial drugs since it is proven or inferred as essential in these pathogens and absent from mammals. Structural studies of IspF from these two important yet distinct pathogens, and comparisons with orthologues have been carried out to generate reagents, to support and inform a structure-based approach to early stage drug discovery.

PubMed: 24410837
DOI: 10.1186/1472-6807-14-1

Experimental method

X-RAY DIFFRACTION (1.9 Å)