4C3C

Thaumatin refined against hatrx data for time-point 1

4C3C の概要

• エントリーDOI: 10.2210/pdb4c3c/pdb
• 分子名称: THAUMATIN-1, L(+)-TARTARIC ACID (3 entities in total)
• 機能のキーワード: plant protein, radiation damage, time-resolved crystallography, hatrx
• 由来する生物種: THAUMATOCOCCUS DANIELLII
• 細胞内の位置: Cytoplasmic vesicle: P02883
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22377.15

構造登録者

Yorke, B.A.,Beddard, G.S.,Owen, R.L.,Pearson, A.R. (登録日: 2013-08-22, 公開日: 2014-09-03, 最終更新日: 2024-11-20)

主引用文献

Yorke, B.A.,Beddard, G.S.,Owen, R.L.,Pearson, A.R.
Time-Resolved Crystallography Using the Hadamard Transform.
Nat.Methods, 11:1131-, 2014

PubMed Abstract: We describe a method for performing time-resolved X-ray crystallographic experiments based on the Hadamard transform, in which time resolution is defined by the underlying periodicity of the probe pulse sequence, and signal/noise is greatly improved over that for the fastest pump-probe experiments depending on a single pulse. This approach should be applicable on standard synchrotron beamlines and will enable high-resolution measurements of protein and small-molecule structural dynamics. It is also applicable to other time-resolved measurements where a probe can be encoded, such as pump-probe spectroscopy.

PubMed: 25282611
DOI: 10.1038/NMETH.3139

実験手法

X-RAY DIFFRACTION (1.8 Å)