4C26
Solution NMR structure of the HicA toxin from Burkholderia pseudomallei
Summary for 4C26
| Entry DOI | 10.2210/pdb4c26/pdb |
| NMR Information | BMRB: 19464 |
| Descriptor | HICA (1 entity in total) |
| Functional Keywords | toxin |
| Biological source | BURKHOLDERIA PSEUDOMALLEI |
| Total number of polymer chains | 1 |
| Total formula weight | 7327.58 |
| Authors | Butt, A.,Higman, V.A.,Williams, C.,Crump, M.P.,Hemsley, C.,Harmer, N.,Titball, R.W. (deposition date: 2013-08-16, release date: 2014-04-23, Last modification date: 2024-05-15) |
| Primary citation | Butt, A.,Higman, V.A.,Williams, C.,Crump, M.P.,Hemsley, C.M.,Harmer, N.,Titball, R.W. The Hica Toxin from Burkholderia Pseudomallei Has a Role in Persister Cell Formation. Biochem.J., 459:333-, 2014 Cited by PubMed Abstract: TA (toxin-antitoxin) systems are widely distributed amongst bacteria and are associated with the formation of antibiotic tolerant (persister) cells that may have involvement in chronic and recurrent disease. We show that overexpression of the Burkholderia pseudomallei HicA toxin causes growth arrest and increases the number of persister cells tolerant to ciprofloxacin or ceftazidime. Furthermore, our data show that persistence towards ciprofloxacin or ceftazidime can be differentially modulated depending on the level of induction of HicA expression. Deleting the hicAB locus from B. pseudomallei K96243 significantly reduced persister cell frequencies following exposure to ciprofloxacin, but not ceftazidime. The structure of HicA(H24A) was solved by NMR and forms a dsRBD-like (dsRNA-binding domain-like) fold, composed of a triple-stranded β-sheet, with two helices packed against one face. The surface of the protein is highly positively charged indicative of an RNA-binding protein and His24 and Gly22 were functionality important residues. This is the first study demonstrating a role for the HicAB system in bacterial persistence and the first structure of a HicA protein that has been experimentally characterized. PubMed: 24502667DOI: 10.1042/BJ20140073 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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