4C16
E-selectin lectin, EGF-like and two SCR domains complexed with glycomimetic antagonist
Summary for 4C16
| Entry DOI | 10.2210/pdb4c16/pdb |
| Related | 4C17 |
| Descriptor | E-SELECTIN, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (8 entities in total) |
| Functional Keywords | cell adhesion, cell-adhesion molecule, c-type lectin, inflammation, leukocyte, glycomimetic, antagonist, catch- bond |
| Biological source | HOMO SAPIENS (HUMAN) |
| Cellular location | Cell membrane ; Single-pass type I membrane protein: P16581 |
| Total number of polymer chains | 2 |
| Total formula weight | 67082.03 |
| Authors | Preston, R.C.,Jakob, R.P.,Binder, F.P.C.,Sager, C.P.,Ernst, B.,Maier, T. (deposition date: 2013-08-09, release date: 2014-08-20, Last modification date: 2024-11-20) |
| Primary citation | Preston, R.C.,Jakob, R.P.,Binder, F.P.,Sager, C.P.,Ernst, B.,Maier, T. E-Selectin Ligand Complexes Adopt an Extended High-Affinity Conformation. J.Mol.Cell.Biol., 8:62-, 2016 Cited by PubMed Abstract: E-selectin is a cell-adhesion molecule of the vascular endothelium that promotes essential leukocyte rolling in the early inflammatory response by binding to glycoproteins containing the tetrasaccharide sialyl Lewis(x) (sLe(x)). Efficient leukocyte recruitment under vascular flow conditions depends on an increased lifetime of E-selectin/ligand complexes under tensile force in a so-called catch-bond binding mode. Co-crystal structures of a representative fragment of the extracellular E-selectin region with sLe(x) and a glycomimetic antagonist thereof reveal an extended E-selectin conformation, which is identified as a high-affinity binding state of E-selectin by molecular dynamics simulations. Small-angle X-ray scattering experiments demonstrate a direct link between ligand binding and E-selectin conformational transition under static conditions in solution. This permits tracing a series of concerted structural changes connecting ligand binding to conformational stretching as the structural basis of E-selectin catch-bond-mediated leukocyte recruitment. The detailed molecular view of the binding site paves the way for the design of a new generation of selectin antagonists. This is of special interest, since their therapeutic potential was recently demonstrated with the pan-selectin antagonists GMI-1070 (Rivipansel). PubMed: 26117840DOI: 10.1093/JMCB/MJV046 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.93 Å) |
Structure validation
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