4C0L

Crystal structure of Drosophila Miro EF hand and cGTPase domains bound to one magnesium ion and Mg:GDP (MgGDP-MiroS)

4C0L の概要

• エントリーDOI: 10.2210/pdb4c0l/pdb
• 関連するPDBエントリー: 4C0J 4C0K
• 分子名称: MITOCHONDRIAL RHO GTPASE, UNKNOWN ATOM OR ION, SULFATE ION, ... (8 entities in total)
• 機能のキーワード: hydrolase, mitochondrial transport, calcium-binding gtpase, kinesin, mitophagy, hidden ef hands
• 由来する生物種: DROSOPHILA MELANOGASTER (FRUIT FLY)
• 細胞内の位置: Mitochondrion outer membrane; Single-pass type IV membrane protein (Probable): Q8IMX7
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50040.64

構造登録者

Klosowiak, J.L.,Focia, P.J.,Wawrzak, Z.,Chakravarthy, S.,Landahl, E.C.,Freymann, D.M.,Rice, S.E. (登録日: 2013-08-05, 公開日: 2013-10-09, 最終更新日: 2024-05-01)

主引用文献

Klosowiak, J.L.,Focia, P.J.,Chakravarthy, S.,Landahl, E.C.,Freymann, D.M.,Rice, S.E.
Structural Coupling of the EF Hand and C-Terminal Gtpase Domains in the Mitochondrial Protein Miro.
Embo Rep., 14:968-, 2013

PubMed Abstract: Miro is a highly conserved calcium-binding GTPase at the regulatory nexus of mitochondrial transport and autophagy. Here we present crystal structures comprising the tandem EF hand and carboxy terminal GTPase (cGTPase) domains of Drosophila Miro. The structures reveal two previously unidentified 'hidden' EF hands, each paired with a canonical EF hand. Each EF hand pair is bound to a helix that structurally mimics an EF hand ligand. A key nucleotide-sensing element and a Pink1 phosphorylation site both lie within an extensive EF hand-cGTPase interface. Our results indicate structural mechanisms for calcium, nucleotide and phosphorylation-dependent regulation of mitochondrial function by Miro.

PubMed: 24071720
DOI: 10.1038/EMBOR.2013.151

実験手法

X-RAY DIFFRACTION (3 Å)