4BWW

Crystal structure of spin labelled azurin T21R1.

4BWW の概要

• エントリーDOI: 10.2210/pdb4bww/pdb
• 分子名称: AZURIN, COPPER (II) ION, GLYCEROL, ... (6 entities in total)
• 機能のキーワード: electron transport
• 由来する生物種: PSEUDOMONAS AERUGINOSA
• 細胞内の位置: Periplasm: P00282
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 59411.05

構造登録者

Hagelueken, G. (登録日: 2013-07-04, 公開日: 2014-06-11, 最終更新日: 2023-12-20)

主引用文献

Florin, N.,Schiemann, O.,Hagelueken, G.
High-Resolution Crystal Structure of Spin Labelled (T21R1) Azurin from Pseudomonas Aeruginosa: A Challenging Structural Benchmark for in Silico Spin Labelling Algorithms.
Bmc Struct.Biol., 14:16-, 2014

PubMed Abstract: EPR-based distance measurements between spin labels in proteins have become a valuable tool in structural biology. The direct translation of the experimental distances into structural information is however often impaired by the intrinsic flexibility of the spin labelled side chains. Different algorithms exist that predict the approximate conformation of the spin label either by using pre-computed rotamer libraries of the labelled side chain (rotamer approach) or by simply determining its accessible volume (accessible volume approach). Surprisingly, comparisons with many experimental distances have shown that both approaches deliver the same distance prediction accuracy of about 3 Å.

PubMed: 24884565
DOI: 10.1186/1472-6807-14-16

実験手法

X-RAY DIFFRACTION (1.48 Å)