4BTP

Structure of the capsid protein P1 of the bacteriophage phi8

4BTP の概要

• エントリーDOI: 10.2210/pdb4btp/pdb
• 分子名称: p1 (1 entity in total)
• 機能のキーワード: viral protein
• 由来する生物種: Pseudomonas phage phi8
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 869991.25

構造登録者

El Omari, K.,Sutton, G.,Ravantti, J.J.,Zhang, H.,Walter, T.S.,Grimes, J.M.,Bamford, D.H.,Stuart, D.I.,Mancini, E.J. (登録日: 2013-06-18, 公開日: 2013-08-07, 最終更新日: 2024-05-08)

主引用文献

El Omari, K.,Sutton, G.,Ravantti, J.J.,Zhang, H.,Walter, T.S.,Grimes, J.M.,Bamford, D.H.,Stuart, D.I.,Mancini, E.J.
Plate Tectonics of Virus Shell Assembly and Reorganization in Phage Phi8, a Distant Relative of Mammalian Reoviruses
Structure, 21:1374-, 2013

PubMed Abstract: The hallmark of a virus is its capsid, which harbors the viral genome and is formed from protein subunits, which assemble following precise geometric rules. dsRNA viruses use an unusual protein multiplicity (120 copies) to form their closed capsids. We have determined the atomic structure of the capsid protein (P1) from the dsRNA cystovirus Φ8. In the crystal P1 forms pentamers, very similar in shape to facets of empty procapsids, suggesting an unexpected assembly pathway that proceeds via a pentameric intermediate. Unlike the elongated proteins used by dsRNA mammalian reoviruses, P1 has a compact trapezoid-like shape and a distinct arrangement in the shell, with two near-identical conformers in nonequivalent structural environments. Nevertheless, structural similarity with the analogous protein from the mammalian viruses suggests a common ancestor. The unusual shape of the molecule may facilitate dramatic capsid expansion during phage maturation, allowing P1 to switch interaction interfaces to provide capsid plasticity.

PubMed: 23891291
DOI: 10.1016/J.STR.2013.06.017

実験手法

X-RAY DIFFRACTION (3.7 Å)