4BS0

Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole

4BS0 の概要

• エントリーDOI: 10.2210/pdb4bs0/pdb
• 分子名称: KEMP ELIMINASE HG3.17, SULFATE ION, 6-NITROBENZOTRIAZOLE, ... (4 entities in total)
• 機能のキーワード: lyase-lyase inhibitor complex, computational protein design, proton transfer, kemp elimination, directed evolution, transition state tuning, bottom-up enzyme construction, elementary chemical step catalysis, lyase/lyase inhibitor
• 由来する生物種: THERMOASCUS AURANTIACUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 69636.14

構造登録者

Blomberg, R.,Kries, H.,Pinkas, D.M.,Mittl, P.R.E.,Gruetter, M.G.,Privett, H.K.,Mayo, S.,Hilvert, D. (登録日: 2013-06-06, 公開日: 2013-10-16, 最終更新日: 2024-10-09)

主引用文献

Blomberg, R.,Kries, H.,Pinkas, D.M.,Mittl, P.R.E.,Gruetter, M.G.,Privett, H.K.,Mayo, S.L.,Hilvert, D.
Precision is Essential for Efficient Catalysis in an Evolved Kemp Eliminase
Nature, 503:418-, 2013

PubMed Abstract: Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of design to one of molecular recognition. Nevertheless, past attempts to capitalize on this idea, for example by using transition state analogues to elicit antibodies with catalytic activities, have generally failed to deliver true enzymatic rates. The advent of computational design approaches, combined with directed evolution, has provided an opportunity to revisit this problem. Starting from a computationally designed catalyst for the Kemp elimination--a well-studied model system for proton transfer from carbon--we show that an artificial enzyme can be evolved that accelerates an elementary chemical reaction 6 × 10(8)-fold, approaching the exceptional efficiency of highly optimized natural enzymes such as triosephosphate isomerase. A 1.09 Å resolution crystal structure of the evolved enzyme indicates that familiar catalytic strategies such as shape complementarity and precisely placed catalytic groups can be successfully harnessed to afford such high rate accelerations, making us optimistic about the prospects of designing more sophisticated catalysts.

PubMed: 24132235
DOI: 10.1038/NATURE12623

実験手法

X-RAY DIFFRACTION (1.09 Å)