4BRZ

Haloalkane dehalogenase

4BRZ の概要

• エントリーDOI: 10.2210/pdb4brz/pdb
• 分子名称: HALOALKANE DEHALOGENASE, CHLORIDE ION (3 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: RHODOBACTERACEAE
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65186.17

構造登録者

Novak, H.R.,Sayer, C.,Isupov, M.,Gotz, D.,Spragg, A.M.,Littlechild, J.A. (登録日: 2013-06-06, 公開日: 2014-05-14, 最終更新日: 2023-12-20)

主引用文献

Novak, H.R.,Sayer, C.,Isupov, M.N.,Gotz, D.,Spragg, A.M.,Littlechild, J.A.
Biochemical and Structural Characterisation of a Haloalkane Dehalogenase from a Marine Rhodobacteraceae.
FEBS Lett., 588:1616-, 2014

PubMed Abstract: A putative haloalkane dehalogenase has been identified in a marine Rhodobacteraceae and subsequently cloned and over-expressed in Escherichia coli. The enzyme has highest activity towards the substrates 1,6-dichlorohexane, 1-bromooctane, 1,3-dibromopropane and 1-bromohexane. The crystal structures of the enzyme in the native and product bound forms reveal a large hydrophobic active site cavity. A deeper substrate binding pocket defines the enzyme preference towards substrates with longer carbon chains. Arg136 at the bottom of the substrate pocket is positioned to bind the distal halogen group of extended di-halogenated substrates.

PubMed: 24613925
DOI: 10.1016/J.FEBSLET.2014.02.056

実験手法

X-RAY DIFFRACTION (1.67 Å)