4BR6

Crystal structure of Chaetomium thermophilum MnSOD

4BR6 の概要

• エントリーDOI: 10.2210/pdb4br6/pdb
• 分子名称: SUPEROXIDE DISMUTASE, MANGANESE (III) ION, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: oxidoreductase, dismutation, antioxidants, thermostability, metal binding
• 由来する生物種: CHAETOMIUM THERMOPHILUM
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 87895.51

構造登録者

Haikarainen, T.,Frioux, C.,Zhnag, L.-Q.,Li, D.-C.,Papageorgiou, A.C. (登録日: 2013-06-04, 公開日: 2013-12-18, 最終更新日: 2023-12-20)

主引用文献

Haikarainen, T.,Frioux, C.,Zhnag, L.,Li, D.,Papageorgiou, A.C.
Crystal Structure and Biochemical Characterization of a Manganese Superoxide Dismutase from Chaetomium Thermophilum.
Biochim.Biophys.Acta, 1844:422-, 2014

PubMed Abstract: A manganese superoxide dismutase from the thermophilic fungus Chaetomium thermophilum (CtMnSOD) was expressed in Pichia pastoris and purified to homogeneity. Its optimal temperature was 60°C with approximately 75% of its activity retained after incubation at 70°C for 60min. Recombinant yeast cells carrying C. thermophilum mnsod gene exhibited higher stress resistance to salt and oxidative stress-inducing agents than control yeast cells. In an effort to provide structural insights, CtMnSOD was crystallized and its structure was determined at 2.0Å resolution. The overall architecture of CtMnSOD was found similar to other MnSODs with highest structural similarities obtained against a MnSOD from the thermotolerant fungus Aspergillus fumigatus. In order to explain its thermostability, structural and sequence analysis of CtMnSOD with other MnSODs was carried out. An increased number of charged residues and an increase in the number of intersubunit salt bridges and the Thr:Ser ratio were identified as potential reasons for the thermostability of CtMnSOD.

PubMed: 24316252
DOI: 10.1016/J.BBAPAP.2013.11.014

実験手法

X-RAY DIFFRACTION (2 Å)