4BON

The structure and super-organization of acetylcholine receptor-rapsyn complexes class B

4BON の概要

• エントリーDOI: 10.2210/pdb4bon/pdb
• 関連するPDBエントリー: 4BOG 4BOI 4BOO
• EMDBエントリー: 2378
• 分子名称: ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA, ACETYLCHOLINE RECEPTOR BETA SUBUNIT, ACETYLCHOLINE RECEPTOR DELTA SUBUNIT, ... (4 entities in total)
• 機能のキーワード: transport protein, neurotransmitter receptor, clustering, synapse, neuromuscular junction, nicotinic, 43k
• 由来する生物種: TORPEDO MARMORATA (MARBLED ELECTRIC RAY); 詳細
• 細胞内の位置: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein: P02711
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 279950.34

構造登録者

Zuber, B.,Unwin, N. (登録日: 2013-05-21, 公開日: 2013-06-26, 最終更新日: 2024-10-09)

主引用文献

Zuber, B.,Unwin, N.
Structure and Superorganization of Acetylcholine Receptor-Rapsyn Complexes.
Proc.Natl.Acad.Sci.USA, 110:10622-, 2013

PubMed Abstract: The scaffolding protein at the neuromuscular junction, rapsyn, enables clustering of nicotinic acetylcholine receptors in high concentration and is critical for muscle function. Patients with insufficient receptor clustering suffer from muscle weakness. However, the detailed organization of the receptor-rapsyn network is poorly understood: it is unclear whether rapsyn first forms a wide meshwork to which receptors can subsequently dock or whether it only forms short bridges linking receptors together to make a large cluster. Furthermore, the number of rapsyn-binding sites per receptor (a heteropentamer) has been controversial. Here, we show by cryoelectron tomography and subtomogram averaging of Torpedo postsynaptic membrane that receptors are connected by up to three rapsyn bridges, the minimum number required to form a 2D network. Half of the receptors belong to rapsyn-connected groups comprising between two and fourteen receptors. Our results provide a structural basis for explaining the stability and low diffusion of receptors within clusters.

PubMed: 23754381
DOI: 10.1073/PNAS.1301277110

実験手法

ELECTRON MICROSCOPY (40 Å)