4BKK

The Respiratory Syncytial Virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid.

4BKK の概要

• エントリーDOI: 10.2210/pdb4bkk/pdb
• EMDBエントリー: 2369
• 分子名称: RNA (161-MER), NUCLEOPROTEIN (2 entities in total)
• 機能のキーワード: viral protein-rna complex, viral protein/rna
• 由来する生物種: HUMAN RESPIRATORY SYNCYTIAL VIRUS A STRAIN LONG; 詳細
• タンパク質・核酸の鎖数: 24
• 化学式量合計: 1049769.73

構造登録者

Bakker, S.E.,Duquerroy, S.,Galloux, M.,Loney, C.,Conner, E.,Eleouet, J.F.,Rey, F.A.,Bhella, D. (登録日: 2013-04-26, 公開日: 2013-10-30, 最終更新日: 2024-05-08)

主引用文献

Bakker, S.E.,Duquerroy, S.,Galloux, M.,Loney, C.,Conner, E.,Eleouet, J.F.,Rey, F.A.,Bhella, D.
The Respiratory Syncytial Virus Nucleoprotein-RNA Complex Forms a Left-Handed Helical Nucleocapsid.
J.Gen.Virol., 94:1734-, 2013

PubMed Abstract: Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. We have previously described the X-ray structure of an NC-like structure: a decameric ring formed of N-RNA that mimics one turn of the helical NC. In the absence of experimental data we had hypothesized that the NC helix would be right-handed, as the N-N contacts in the ring appeared to more easily adapt to that conformation. We now unambiguously show that the RSV NC is a left-handed helix. We further show that the contacts in the ring can be distorted to maintain key N-N-protein interactions in a left-handed helix, and discuss the implications of the resulting atomic model of the helical NC for viral replication and transcription.

PubMed: 23677789
DOI: 10.1099/VIR.0.053025-0

実験手法

ELECTRON MICROSCOPY