4BK0

Crystal structure of the KIX domain of human RECQL5 (domain-swapped dimer)

4BK0 の概要

• エントリーDOI: 10.2210/pdb4bk0/pdb
• EMDBエントリー: 2367
• 分子名称: ATP-DEPENDENT DNA HELICASE Q5, DI(HYDROXYETHYL)ETHER (3 entities in total)
• 機能のキーワード: transcription, dna helicase, transcriptional repression
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25252.74

構造登録者

Kassube, S.A.,Jinek, M.,Fang, J.,Tsutakawa, S.,Nogales, E. (登録日: 2013-04-21, 公開日: 2013-06-12, 最終更新日: 2024-05-08)

主引用文献

Kassube, S.A.,Jinek, M.,Fang, J.,Tsutakawa, S.,Nogales, E.
Structural Mimicry in Transcription Regulation of Human RNA Polymerase II by the DNA Helicase Recql5
Nat.Struct.Mol.Biol., 20:892-, 2013

PubMed Abstract: RECQL5 is a member of the highly conserved RecQ family of DNA helicases involved in DNA repair. RECQL5 interacts with RNA polymerase II (Pol II) and inhibits transcription of protein-encoding genes by an unknown mechanism. We show that RECQL5 contacts the Rpb1 jaw domain of Pol II at a site that overlaps with the binding site for the transcription elongation factor TFIIS. Our cryo-EM structure of elongating Pol II arrested in complex with RECQL5 shows that the RECQL5 helicase domain is positioned to sterically block elongation. The crystal structure of the RECQL5 KIX domain reveals similarities with TFIIS, and binding of RECQL5 to Pol II interferes with the ability of TFIIS to promote transcriptional read-through in vitro. Together, our findings reveal a dual mode of transcriptional repression by RECQL5 that includes structural mimicry of the Pol II-TFIIS interaction.

PubMed: 23748380
DOI: 10.1038/NSMB.2596

実験手法

X-RAY DIFFRACTION (1.9 Å)