4BJP

Crystal structure of E. coli penicillin binding protein 3

4BJP の概要

• エントリーDOI: 10.2210/pdb4bjp/pdb
• 関連するPDBエントリー: 4BJQ
• 分子名称: PENICILLIN BINDING PROTEIN TRANSPEPTIDASE DOMAIN PROTEIN, SULFATE ION, 3-CYCLOHEXYL-1-PROPYLSULFONIC ACID, ... (7 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 58095.50

構造登録者

Sauvage, E.,Joris, M.,Herman, R.,Kerff, F.,Rocaboy, M.,Charlier, P. (登録日: 2013-04-19, 公開日: 2014-05-07, 最終更新日: 2023-12-20)

主引用文献

Sauvage, E.,Derouaux, A.,Fraipont, C.,Joris, M.,Herman, R.,Rocaboy, M.,Schloesser, M.,Dumas, J.,Kerff, F.,Nguyen-Disteche, M.,Charlier, P.
Crystal Structure of Penicillin-Binding Protein 3 (Pbp3) from Escherichia Coli.
Plos One, 9:98042-, 2014

PubMed Abstract: In Escherichia coli, penicillin-binding protein 3 (PBP3), also known as FtsI, is a central component of the divisome, catalyzing cross-linking of the cell wall peptidoglycan during cell division. PBP3 is mainly periplasmic, with a 23 residues cytoplasmic tail and a single transmembrane helix. We have solved the crystal structure of a soluble form of PBP3 (PBP3(57-577)) at 2.5 Å revealing the two modules of high molecular weight class B PBPs, a carboxy terminal module exhibiting transpeptidase activity and an amino terminal module of unknown function. To gain additional insight, the PBP3 Val88-Ser165 subdomain (PBP3(88-165)), for which the electron density is poorly defined in the PBP3 crystal, was produced and its structure solved by SAD phasing at 2.1 Å. The structure shows a three dimensional domain swapping with a β-strand of one molecule inserted between two strands of the paired molecule, suggesting a possible role in PBP3(57-577) dimerization.

PubMed: 24875494
DOI: 10.1371/JOURNAL.PONE.0098042

実験手法

X-RAY DIFFRACTION (2.5 Å)