4BIG

Crystal structure of the conserved staphylococcal antigen 1B, Csa1B

4BIG の概要

• エントリーDOI: 10.2210/pdb4big/pdb
• 関連するPDBエントリー: 4BIH
• 分子名称: UNCHARACTERIZED LIPOPROTEIN SAOUHSC_00053 (2 entities in total)
• 機能のキーワード: immune system, domain of unknown function 576 (duf576), tandem lipoprotein, protective immunity
• 由来する生物種: STAPHYLOCOCCUS AUREUS
• 細胞内の位置: Cell membrane; Lipid-anchor (Potential): Q2G1Q0
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29462.05

構造登録者

Malito, E.,Bottomley, M.J.,Schluepen, C.,Liberatori, S. (登録日: 2013-04-10, 公開日: 2013-08-07, 最終更新日: 2024-10-16)

主引用文献

Schluepen, C.,Malito, E.,Marongiu, A.,Schirle, M.,Mcwhinnie, E.,Lo Surdo, P.,Biancucci, M.,Falugi, F.,Nardi-Dei, V.,Marchi, S.,Fontana, M.R.,Lombardi, B.,De Falco, M.G.,Rinaudo, C.D.,Spraggon, G.,Nissum, M.,Bagnoli, F.,Grandi, G.,Bottomley, M.J.,Liberatori, S.
Mining the Bacterial Unknown Proteome: Identification and Characterization of a Novel Family of Highly Conserved Protective Antigens in Staphylococcus Aureus
Biochem.J., 455:273-, 2013

PubMed Abstract: In the human pathogen Staphylococcus aureus, there exists an enormous diversity of proteins containing DUFs (domains of unknown function). In the present study, we characterized the family of conserved staphylococcal antigens (Csa) classified as DUF576 and taxonomically restricted to Staphylococci. The 18 Csa paralogues in S. aureus Newman are highly similar at the sequence level, yet were found to be expressed in multiple cellular locations. Extracellular Csa1A was shown to be post-translationally processed and released. Molecular interaction studies revealed that Csa1A interacts with other Csa paralogues, suggesting that these proteins are involved in the same cellular process. The structures of Csa1A and Csa1B were determined by X-ray crystallography, unveiling a peculiar structure with limited structural similarity to other known proteins. Our results provide the first detailed biological characterization of this family and confirm the uniqueness of this family also at the structural level. We also provide evidence that Csa family members elicit protective immunity in in vivo animal models of staphylococcal infections, indicating a possible important role for these proteins in S. aureus biology and pathogenesis. These findings identify the Csa family as new potential vaccine candidates, and underline the importance of mining the bacterial unknown proteome to identify new targets for preventive vaccines.

PubMed: 23895222
DOI: 10.1042/BJ20130540

実験手法

X-RAY DIFFRACTION (2.274 Å)