4BHL

Crystal structure of Litopenaeus vannamei arginine kinase in binary complex with arginine

4BHL の概要

• エントリーDOI: 10.2210/pdb4bhl/pdb
• 分子名称: ARGININE KINASE, ARGININE, BETA-MERCAPTOETHANOL, ... (4 entities in total)
• 機能のキーワード: transferase, binary complex, phosphagen
• 由来する生物種: LITOPENAEUS VANNAMEI (PACIFIC WHITE SHRIMP)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40461.07

構造登録者

Lopez-Zavala, A.A.,Garcia-Orozco, K.D.,Carrasco-Miranda, J.S.,Hernandez-Flores, J.M.,Sugich-Miranda, R.,Velazquez-Contreras, E.F.,Criscitiello, M.F.,Brieba, L.G.,Rudino-Pinera, E.,Sotelo-Mundo, R.R. (登録日: 2013-04-03, 公開日: 2013-09-04, 最終更新日: 2023-12-20)

主引用文献

Lopez-Zavala, A.A.,Garcia-Orozco, K.D.,Carrasco-Miranda, J.S.,Sugich-Miranda, R.,Velazquez-Contreras, E.F.,Criscitiello, M.F.,Brieba, L.G.,Rudino-Pinera, E.,Sotelo-Mundo, R.R.
Crystal Structure of Shrimp Arginine Kinase in Binary Complex with Arginine-A Molecular View of the Phosphagen Precursor Binding to the Enzyme.
J.Bioenerg.Biomembr., 45:511-, 2013

PubMed Abstract: Arginine kinase (AK) is a key enzyme for energetic balance in invertebrates. Although AK is a well-studied system that provides fast energy to invertebrates using the phosphagen phospho-arginine, the structural details on the AK-arginine binary complex interaction remain unclear. Herein, we determined two crystal structures of the Pacific whiteleg shrimp (Litopenaeus vannamei) arginine kinase, one in binary complex with arginine (LvAK-Arg) and a ternary transition state analog complex (TSAC). We found that the arginine guanidinium group makes ionic contacts with Glu225, Cys271 and a network of ordered water molecules. On the zwitterionic side of the amino acid, the backbone amide nitrogens of Gly64 and Val65 coordinate the arginine carboxylate. Glu314, one of proposed acid-base catalytic residues, did not interact with arginine in the binary complex. This residue is located in the flexible loop 310-320 that covers the active site and only stabilizes in the LvAK-TSAC. This is the first binary complex crystal structure of a guanidine kinase in complex with the guanidine substrate and could give insights into the nature of the early steps of phosphagen biosynthesis.

PubMed: 23873077
DOI: 10.1007/S10863-013-9521-0

実験手法

X-RAY DIFFRACTION (1.9 Å)