4BG8

Apo form of a putative sugar kinase MK0840 from Methanopyrus kandleri (monoclinic space group)

4BG8 の概要

• エントリーDOI: 10.2210/pdb4bg8/pdb
• 関連するPDBエントリー: 4BG9 4BGA 4BGB
• 分子名称: PREDICTED MOLECULAR CHAPERONE DISTANTLY RELATED TO HSP70-F OLD METALLOPROTEASES, POTASSIUM ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: transferase, askha superfamily, phosphotransfer, pseudomurein
• 由来する生物種: METHANOPYRUS KANDLERI
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 78376.29

構造登録者

Schacherl, M.,Waltersperger, S.M.,Baumann, U. (登録日: 2013-03-24, 公開日: 2013-11-27, 最終更新日: 2024-11-06)

主引用文献

Schacherl, M.,Waltersperger, S.M.,Baumann, U.
Structural Characterization of the Ribonuclease H-Like Type Askha Superfamily Kinase Mk0840 from Methanopyrus Kandleri
Acta Crystallogr.,Sect.D, 69:2440-, 2013

PubMed Abstract: Murein recycling is a process in which microorganisms recover peptidoglycan-degradation products in order to utilize them in cell wall biosynthesis or basic metabolic pathways. Methanogens such as Methanopyrus kandleri contain pseudomurein, which differs from bacterial murein in its composition and branching. Here, four crystal structures of the putative sugar kinase MK0840 from M. kandleri in apo and nucleotide-bound states are reported. MK0840 shows high similarity to bacterial anhydro-N-acetylmuramic acid kinase, which is involved in murein recycling. The structure shares a common fold with panthothenate kinase and the 2-hydroxyglutaryl-CoA dehydratase component A, both of which are members of the ASKHA (acetate and sugar kinases/Hsc70/actin) superfamily of phosphotransferases. Local conformational changes in the nucleotide-binding site between the apo and holo forms are observed upon nucleotide binding. Further insight is given into domain movements and putative active-site residues are identified.

PubMed: 24311585
DOI: 10.1107/S0907444913022683

実験手法

X-RAY DIFFRACTION (1.96 Å)