4BG7

Bacteriophage T5 Homolog of the Eukaryotic Transcription Coactivator PC4 Implicated in Recombination-Dependent DNA Replication

4BG7 の概要

• エントリーDOI: 10.2210/pdb4bg7/pdb
• 分子名称: PUTATIVE TRANSCRIPTIONAL COACTIVATOR P15 (2 entities in total)
• 機能のキーワード: replication, recombination, repair, transcription, ssb
• 由来する生物種: ENTEROBACTERIA PHAGE T5 (BACTERIOPHAGE T5)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24108.72

構造登録者

Steigemann, B.,Schulz, A.,Hinrichs, W.,Werten, S. (登録日: 2013-03-24, 公開日: 2013-09-11, 最終更新日: 2024-05-08)

主引用文献

Steigemann, B.,Schulz, A.,Werten, S.
Bacteriophage T5 Encodes a Homolog of the Eukaryotic Transcription Coactivator Pc4 Implicated in Recombination-Dependent DNA Replication.
J.Mol.Biol., 425:4125-, 2013

PubMed Abstract: The RNA polymerase II cofactor PC4 globally regulates transcription of protein-encoding genes through interactions with unwinding DNA, the basal transcription machinery and transcription activators. Here, we report the surprising identification of PC4 homologs in all sequenced representatives of the T5 family of bacteriophages, as well as in an archaeon and seven phyla of eubacteria. We have solved the crystal structure of the full-length T5 protein at 1.9Å, revealing a striking resemblance to the characteristic single-stranded DNA (ssDNA)-binding core domain of PC4. Intriguing novel structural features include a potential regulatory region at the N-terminus and a C-terminal extension of the homodimerisation interface. The genome organisation of T5-related bacteriophages points at involvement of the PC4 homolog in recombination-dependent DNA replication, strongly suggesting that the protein corresponds to the hitherto elusive replicative ssDNA-binding protein of the T5 family. Our findings imply that PC4-like factors intervene in multiple unwinding-related processes by acting as versatile modifiers of nucleic acid conformation and raise the possibility that the eukaryotic transcription coactivator derives from ancestral DNA replication, recombination and repair factors.

PubMed: 24029071
DOI: 10.1016/J.JMB.2013.09.001

実験手法

X-RAY DIFFRACTION (1.9 Å)