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4BFN

Crystal Structure of the Starch-Binding Domain from Rhizopus oryzae Glucoamylase in Complex with isomaltotetraose

Summary for 4BFN
Entry DOI10.2210/pdb4bfn/pdb
Related4BFO
DescriptorGLUCOAMYLASE, alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose (3 entities in total)
Functional Keywordshydrolase, carbohydrate binding
Biological sourceRHIZOPUS ORYZAE
Total number of polymer chains1
Total formula weight12329.16
Authors
Chu, C.H.,Li, K.M.,Lin, S.W.,Sun, Y.J. (deposition date: 2013-03-21, release date: 2013-10-23, Last modification date: 2023-12-20)
Primary citationChu, C.,Li, K.,Lin, S.,Chang, M.D.,Jiang, T.,Sun, Y.
Crystal Structures of Starch Binding Domain from Rhizopus Oryzae Glucoamylase in Complex with Isomaltooligosaccharide: Insights Into Polysaccharide Binding Mechanism of Cbm21 Family.
Proteins, 82:1079-, 2014
Cited by
PubMed Abstract: Glucoamylases are responsible for hydrolysis of starch and polysaccharides to yield β-D-glucose. Rhizopus oryzae glucoamylase (RoGA) is composed of an N-terminal starch binding domain (SBD) and a C-terminal catalytic domain connected by an O-glycosylated linker. Two carbohydrate binding sites in RoSBD have been identified, site I is created by three highly conserved aromatic residues, Trp47, Tyr83, and Tyr94, and site II is built up by Tyr32 and Phe58. Here, the two crystal structures of RoSBD in complex with only α-(1,6)-linked isomaltotriose (RoSBD-isoG3) and isomaltotetraose (RoSBD-isoG4) have been determined at 1.2 and 1.3 Å, respectively. Interestingly, site II binding is observed in both complexes, while site I binding is only found in the RoSBD-isoG4 complex. Hence, site II acts as the recognition binding site for carbohydrate and site I accommodates site II to bind isoG4. Site I participates in sugar binding only when the number of glucosyl units of oligosaccharides is more than three. Taken together, two carbohydrate binding sites in RoSBD cooperate to reinforce binding mode of glucoamylase with polysaccharides as well as the starch.
PubMed: 24108499
DOI: 10.1002/PROT.24446
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.32 Å)
Structure validation

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数据于2024-10-30公开中

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