4BAR

Thaumatin from Thaumatococcus daniellii structure in complex with the europium tris-hydroxyethyltriazoledipicolinate complex at 1.20 A resolution.

4BAR の概要

• エントリーDOI: 10.2210/pdb4bar/pdb
• 関連するPDBエントリー: 4BAL
• 分子名称: THAUMATIN-1, 4-(4-(2-hydroxyethyl)-1H-1,2,3-triazol-1-yl)pyridine-2,6-dicarboxylic acid, EUROPIUM (III) ION, ... (4 entities in total)
• 機能のキーワード: plant protein, click-chemistry, de novo phasing, experimental phasing, lanthanide complex, anomalous scattering
• 由来する生物種: THAUMATOCOCCUS DANIELLII (KATEMFE)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23219.73

構造登録者

Talon, R.,Kahn, R.,Gautier, A.,Nauton, L.,Girard, E. (登録日: 2012-09-14, 公開日: 2012-11-14, 最終更新日: 2024-11-20)

主引用文献

Talon, R.,Nauton, L.,Canet, J.L.,Kahn, R.,Girard, E.,Gautier, A.
Clicked europium dipicolinate complexes for protein X-ray structure determination.
Chem. Commun. (Camb.), 48:11886-11888, 2012

PubMed Abstract: New trisdipicolinic acid-lanthanide complexes are reported as phasing agents for X-ray crystallography of proteins. It is demonstrated that CuAAC modifications allow protein co-crystallization with low concentration of lanthanide complexes leading to an accurate structure determination.

PubMed: 23123834
DOI: 10.1039/c2cc36982f

実験手法

X-RAY DIFFRACTION (1.2 Å)