4BAJ

MYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laser

Summary for 4BAJ

Entry DOI	10.2210/pdb4baj/pdb
Related	1ZTB 4BAI
Descriptor	CHORISMATE SYNTHASE, ACETATE ION (3 entities in total)
Functional Keywords	lyase
Biological source	MYCOBACTERIUM TUBERCULOSIS
Total number of polymer chains	1
Total formula weight	43307.18
Authors	Pereira, P.J.B.,Royant, A.,Panjikar, S.,de Sanctis, D. (deposition date: 2012-09-14, release date: 2013-04-17, Last modification date: 2024-10-16)
Primary citation	Pereira, P.J.,Royant, A.,Panjikar, S.,de Sanctis, D. In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures. J. Struct. Biol., 181:89-94, 2013 Cited by PubMed Abstract: Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation-damage-induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins. PubMed: 23178456 DOI: 10.1016/j.jsb.2012.11.003 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.3 Å)

Structure validation