4BAJ

MYCOBACTERIUM TUBERCULOSIS CHORISMATE SYNTHASE after exposure to 266nm UV laser

4BAJ の概要

• エントリーDOI: 10.2210/pdb4baj/pdb
• 関連するPDBエントリー: 1ZTB 4BAI
• 分子名称: CHORISMATE SYNTHASE, ACETATE ION (3 entities in total)
• 機能のキーワード: lyase
• 由来する生物種: MYCOBACTERIUM TUBERCULOSIS
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43307.18

構造登録者

Pereira, P.J.B.,Royant, A.,Panjikar, S.,de Sanctis, D. (登録日: 2012-09-14, 公開日: 2013-04-17, 最終更新日: 2024-10-16)

主引用文献

Pereira, P.J.,Royant, A.,Panjikar, S.,de Sanctis, D.
In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures.
J. Struct. Biol., 181:89-94, 2013

PubMed Abstract: Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation-damage-induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.

PubMed: 23178456
DOI: 10.1016/j.jsb.2012.11.003

実験手法

X-RAY DIFFRACTION (2.3 Å)