4BAI

Mycobacterium tuberculosis Chorismate synthase before exposure to 266 nm UV laser

Summary for 4BAI

• Entry DOI: 10.2210/pdb4bai/pdb
• Related: 1ZTB 4BAJ
• Descriptor: CHORISMATE SYNTHASE, ACETATE ION (3 entities in total)
• Functional Keywords: lyase
• Biological source: MYCOBACTERIUM TUBERCULOSIS
• Total number of polymer chains: 1
• Total formula weight: 43307.18

Authors

Pereira, P.J.B.,Royant, A.,Panjikar, S.,de Sanctis, D. (deposition date: 2012-09-14, release date: 2013-04-17, Last modification date: 2024-10-16)

Primary citation

Pereira, P.J.,Royant, A.,Panjikar, S.,de Sanctis, D.
In-house UV radiation-damage-induced phasing of selenomethionine-labeled protein structures.
J. Struct. Biol., 181:89-94, 2013

PubMed Abstract: Selenomethionine labeling is the most common technique used in protein crystallography to derivatize recombinant proteins for experimental phasing using anomalous scattering at tunable synchrotron beamlines. Recently, it has been shown that UV radiation depletes electron density of selenium atoms of selenomethionine residues and that UV radiation-damage-induced phasing (equivalent to single isomorphous replacement) protocol can be applied to calculate experimental phases. Here we present the straightforward integration of a UV source with an in-house diffractometer. We show how this setup can extend the capabilities of a sealed tube X-ray generator and be used for experimental phasing of selenium-labeled proteins.

PubMed: 23178456
DOI: 10.1016/j.jsb.2012.11.003

Experimental method

X-RAY DIFFRACTION (2.3 Å)