4BAC

prototype foamy virus strand transfer complexes on product DNA

Summary for 4BAC

• Entry DOI: 10.2210/pdb4bac/pdb
• Related: 2X6N 2X6S 2X74 2X78
• Descriptor: INTEGRASE, 5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP *TP*TP*GP*TP*AP)-3', DNA (38-MER), ... (7 entities in total)
• Functional Keywords: transferase-dna complex, transferase/dna
• Biological source: HUMAN SPUMARETROVIRUS; More
• Total number of polymer chains: 5
• Total formula weight: 112140.63

Authors

Yin, Z.,Lapkouski, M.,Yang, W.,Craigie, R. (deposition date: 2012-09-12, release date: 2012-10-10, Last modification date: 2023-12-20)

Primary citation

Yin, Z.,Lapkouski, M.,Yang, W.,Craigie, R.
Assembly of Prototype Foamy Virus Strand Transfer Complexes on Product DNA Bypassing Catalysis of Integration.
Protein Sci., 21:1849-, 2012

PubMed Abstract: Integrase is the key enzyme that mediates integration of retroviral DNA into cellular DNA which is essential for viral replication. Inhibitors of HIV-1 that target integrase recognize the nucleoprotein complexes formed by integrase and viral DNA substrate (intasomes) rather than the free enzyme. Atomic resolution structures of HIV-1 intasomes are therefore required to understand the mechanisms of inhibition and drug resistance. To date, prototype foamy virus (PFV) is the only retrovirus for which such structures have been determined. We show that PFV strand transfer complexes (STC) can be assembled on product DNA without going through the normal forward reaction pathway. The finding that a retroviral STC can be assembled in this way may provide a powerful tool to alleviate the obstacles that impede structural studies of nucleoprotein intermediates in HIV-1 DNA integration.

PubMed: 23011895
DOI: 10.1002/PRO.2166

Experimental method

X-RAY DIFFRACTION (3.263 Å)