4BAC

prototype foamy virus strand transfer complexes on product DNA

4BAC の概要

• エントリーDOI: 10.2210/pdb4bac/pdb
• 関連するPDBエントリー: 2X6N 2X6S 2X74 2X78
• 分子名称: INTEGRASE, 5'-D(*AP*TP*TP*GP*TP*CP*AP*TP*GP*GP*AP*AP*TP*TP *TP*TP*GP*TP*AP)-3', DNA (38-MER), ... (7 entities in total)
• 機能のキーワード: transferase-dna complex, transferase/dna
• 由来する生物種: HUMAN SPUMARETROVIRUS; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 112140.63

構造登録者

Yin, Z.,Lapkouski, M.,Yang, W.,Craigie, R. (登録日: 2012-09-12, 公開日: 2012-10-10, 最終更新日: 2023-12-20)

主引用文献

Yin, Z.,Lapkouski, M.,Yang, W.,Craigie, R.
Assembly of Prototype Foamy Virus Strand Transfer Complexes on Product DNA Bypassing Catalysis of Integration.
Protein Sci., 21:1849-, 2012

PubMed Abstract: Integrase is the key enzyme that mediates integration of retroviral DNA into cellular DNA which is essential for viral replication. Inhibitors of HIV-1 that target integrase recognize the nucleoprotein complexes formed by integrase and viral DNA substrate (intasomes) rather than the free enzyme. Atomic resolution structures of HIV-1 intasomes are therefore required to understand the mechanisms of inhibition and drug resistance. To date, prototype foamy virus (PFV) is the only retrovirus for which such structures have been determined. We show that PFV strand transfer complexes (STC) can be assembled on product DNA without going through the normal forward reaction pathway. The finding that a retroviral STC can be assembled in this way may provide a powerful tool to alleviate the obstacles that impede structural studies of nucleoprotein intermediates in HIV-1 DNA integration.

PubMed: 23011895
DOI: 10.1002/PRO.2166

実験手法

X-RAY DIFFRACTION (3.263 Å)