4B9R

Crystal structure of the Major Birch Pollen Allergen Bet v 1.0101 (isoform a) nitrated in vitro with tetranitromethan.

4B9R の概要

• エントリーDOI: 10.2210/pdb4b9r/pdb
• 関連するPDBエントリー: 1B6F 1BTV 1BV1 1FSK 1LLT 1QMR 4A80 4A81 4A83 4A84 4A85 4A86 4A87 4A88 4A8G
• 分子名称: MAJOR POLLEN ALLERGEN BET V 1-A, SULFATE ION (3 entities in total)
• 機能のキーワード: allergen, nitration, nitrotyrosine
• 由来する生物種: BETULA PENDULA (EUROPEAN WHITE BIRCH)
• 細胞内の位置: Cytoplasm: P15494
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17737.64

構造登録者

Kofler, S.,Ackaert, C.,Brandstetter, H. (登録日: 2012-09-06, 公開日: 2013-10-16, 最終更新日: 2023-12-20)

主引用文献

Ackaert, C.,Kofler, S.,Horejs-Hoeck, J.,Zulehner, N.,Asam, C.,Von Grafenstein, S.,Fuchs, J.E.,Briza, P.,Liedl, K.R.,Bohle, B.,Ferreira, F.,Brandstetter, H.,Oostingh, G.J.,Duschl, A.
The Impact of Nitration on the Structure and Immunogenicity of the Major Birch Pollen Allergen Bet V 1.0101.
Plos One, 9:4520-, 2014

PubMed Abstract: Allergy prevalence has increased in industrialized countries. One contributing factor could be pollution, which can cause nitration of allergens exogenously (in the air) or endogenously (in inflamed lung tissue). We investigated the impact of nitration on both the structural and immunological behavior of the major birch pollen allergen Bet v 1.0101 to determine whether nitration might be a factor in the increased incidence of allergy. Bet v 1.0101 was nitrated with tetranitromethane. Immune effects were assessed by measuring the proliferation of specific T-cell lines (TCLs) upon stimulation with different concentrations of nitrated and unmodified allergen, and by measurement of cytokine release of monocyte-derived dendritic cells (moDCs) and primary DCs (primDCs) stimulated with nitrated versus unmodified allergen. HPLC-MS, crystallography, gel electrophoresis, amino acid analysis, size exclusion chromatography and molecular dynamics simulation were performed to characterize structural changes after nitration of the allergen. The proliferation of specific TCLs was higher upon stimulation with the nitrated allergen in comparison to the unmodified allergen. An important structural consequence of nitration was oligomerization. Moreover, analysis of the crystal structure of nitrated Bet v 1.0101 showed that amino acid residue Y83, located in the hydrophobic cavity, was nitrated to 100%. Both moDCs and primDCs showed decreased production of TH1-priming cytokines, thus favoring a TH2 response. These results implicate that nitration of Bet v 1.0101 might be a contributing factor to the observed increase in birch pollen allergy, and emphasize the importance of protein modifications in understanding the molecular basis of allergenicity.

PubMed: 25126882
DOI: 10.1371/JOURNAL.PONE.0104520

実験手法

X-RAY DIFFRACTION (1.76 Å)