4B8T

RNA BINDING PROTEIN Solution structure of the third KH domain of KSRP in complex with the G-rich target sequence.

4B8T の概要

• エントリーDOI: 10.2210/pdb4b8t/pdb
• NMR情報: BMRB: 18702
• 分子名称: KH-TYPE SPLICING REGULATORY PROTEIN, 5'-R(*AP*GP*GP*GP*UP)-3' (2 entities in total)
• 機能のキーワード: transcription-rna complex, transcription/rna
• 由来する生物種: HOMO SAPIENS (HUMAN); 詳細
• 細胞内の位置: Nucleus: Q92945
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12538.50

構造登録者

Nicastro, G.,Garcia-Mayoral, M.F.,Hollingworth, D.,Kelly, G.,Martin, S.R.,Briata, P.,Gherzi, R.,Ramos, A. (登録日: 2012-08-30, 公開日: 2012-11-07, 最終更新日: 2024-05-15)

主引用文献

Nicastro, G.,Garcia-Mayoral, M.F.,Hollingworth, D.,Kelly, G.,Martin, S.R.,Briata, P.,Gherzi, R.,Ramos, A.
Noncanonical G Recognition Mediates Ksrp Regulation of Let-7 Biogenesis
Nat.Struct.Mol.Biol., 19:1282-, 2012

PubMed Abstract: Let-7 is an important tumor-suppressive microRNA (miRNA) that acts as an on-off switch for cellular differentiation and regulates the expression of a set of human oncogenes. Binding of the human KSRP protein to let-7 miRNA precursors positively regulates their processing to mature let-7, thereby contributing to control of cell proliferation, apoptosis and differentiation. Here we analyze the molecular basis for KSRP-let-7 precursor selectivity and show how the third KH domain of the protein recognizes a G-rich sequence in the pre-let-7 terminal loop and dominates the interaction. The structure of the KH3-RNA complex explains the protein recognition of this noncanonical KH target sequence, and we demonstrate that the specificity of this binding is crucial for the functional interaction between the protein and the miRNA precursor.

PubMed: 23142982
DOI: 10.1038/NSMB.2427

実験手法

SOLUTION NMR