4B8D

TENSEGRITY TRIANGLE FROM ENZYMATICALLY MANUFACTURED DNA

4B8D の概要

• エントリーDOI: 10.2210/pdb4b8d/pdb
• 分子名称: 5'-D(*GP*AP*GP*CP*AP*GP*CP*CP*TP*GP*TP*AP*CP*DGP *GP*AP*CP*AP*TP*CP*A)-3', 5'-D(*CP*CP*GP*TP*AP*CP*AP)-3', 5'-D(*GP*GP*CP*TP*GP*CP)-3', ... (4 entities in total)
• 機能のキーワード: dna, rolling circle amplification, dna origami
• 由来する生物種: SYNTHETIC CONSTRUCT; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 12797.42

構造登録者

Ducani, C.,Kaul, C.D.,Moche, M.,Shih, W.M.,Hogberg, B. (登録日: 2012-08-26, 公開日: 2013-06-05, 最終更新日: 2023-12-20)

主引用文献

Ducani, C.,Kaul, C.D.,Moche, M.,Shih, W.M.,Hogberg, B.
Enzymatic Production of 'Monoclonal Stoichiometric' Single-Stranded DNA Oligonucleotides
Nat.Methods, 10:647-, 2013

PubMed Abstract: Single-stranded oligonucleotides are important as research tools, as diagnostic probes, in gene therapy and in DNA nanotechnology. Oligonucleotides are typically produced via solid-phase synthesis, using polymer chemistries that are limited relative to what biological systems produce. The number of errors in synthetic DNA increases with oligonucleotide length, and the resulting diversity of sequences can be a problem. Here we present the 'monoclonal stoichiometric' (MOSIC) method for enzyme-mediated production of DNA oligonucleotides. We amplified oligonucleotides from clonal templates derived from single bacterial colonies and then digested cutter hairpins in the products, which released pools of oligonucleotides with precisely controlled relative stoichiometric ratios. We prepared 14-378-nucleotide MOSIC oligonucleotides either by in vitro rolling-circle amplification or by amplification of phagemid DNA in Escherichia coli. Analyses of the formation of a DNA crystal and folding of DNA nanostructures confirmed the scalability, purity and stoichiometry of the produced oligonucleotides.

PubMed: 23727986
DOI: 10.1038/NMETH.2503

実験手法

X-RAY DIFFRACTION (4.79 Å)