4B4E

1.00 A Structure of Lysozyme Crystallized with (R)-2-methyl-2,4- pentanediol

Summary for 4B4E

• Entry DOI: 10.2210/pdb4b4e/pdb
• Related: 4B49 4B4I 4B4J
• Descriptor: LYSOZYME C, (4R)-2-METHYLPENTANE-2,4-DIOL, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: hydrolase, chirality
• Biological source: GALLUS GALLUS (CHICKEN)
• Total number of polymer chains: 1
• Total formula weight: 14673.87

Authors

Jakoncic, J.,Berger, J.,Stauber, M.,Axelbaum, A.,Asherie, N. (deposition date: 2012-07-30, release date: 2012-08-22, Last modification date: 2024-11-13)

Primary citation

Stauber, M.,Jakoncic, J.,Berger, J.,Karp, J.M.,Axelbaum, A.,Sastow, D.,Buldyrev, S.V.,Hrnjez, B.J.,Asherie, N.
Crystallization of Lysozyme with (R)-, (S)- and (Rs)-2-Methyl-2,4-Pentanediol
Acta Crystallogr.,Sect.D, 71:427-, 2015

PubMed Abstract: Chiral control of crystallization has ample precedent in the small-molecule world, but relatively little is known about the role of chirality in protein crystallization. In this study, lysozyme was crystallized in the presence of the chiral additive 2-methyl-2,4-pentanediol (MPD) separately using the R and S enantiomers as well as with a racemic RS mixture. Crystals grown with (R)-MPD had the most order and produced the highest resolution protein structures. This result is consistent with the observation that in the crystals grown with (R)-MPD and (RS)-MPD the crystal contacts are made by (R)-MPD, demonstrating that there is preferential interaction between lysozyme and this enantiomer. These findings suggest that chiral interactions are important in protein crystallization.

PubMed: 25760593
DOI: 10.1107/S1399004714025061

Experimental method

X-RAY DIFFRACTION (1 Å)