4B1Q

NMR structure of the glycosylated conotoxin CcTx from Conus consors

4B1Q の概要

• エントリーDOI: 10.2210/pdb4b1q/pdb
• NMR情報: BMRB: 18897
• 分子名称: CONOTOXIN CCTX, alpha-L-galactopyranose-(1-2)-beta-D-galactopyranose-(1-3)-[alpha-L-galactopyranose-(1-4)-2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-6)]2-acetamido-2-deoxy-alpha-D-galactopyranose (2 entities in total)
• 機能のキーワード: toxin, o-glycan
• 由来する生物種: CONUS CONSORS
• 細胞内の位置: Secreted: P58928
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4142.57

構造登録者

Hocking, H.G.,Gerwig, G.J.,Favreau, P.,Stocklin, R.,Kamerling, J.P.,Boelens, R. (登録日: 2012-07-12, 公開日: 2013-02-06, 最終更新日: 2025-04-09)

主引用文献

Hocking, H.G.,Gerwig, G.J.,Dutertre, S.,Violette, A.,Favreau, P.,Stocklin, R.,Kamerling, J.P.,Boelens, R.
Structure of the O-Glycosylated Conopeptide Cctx from Conus Consors Venom.
Chemistry, 19:870-, 2013

PubMed Abstract: The glycopeptide CcTx, isolated from the venom of the piscivorous cone snail Conus consors, belongs to the κA-family of conopeptides. These toxins elicit excitotoxic responses in the prey by acting on voltage-gated sodium channels. The structure of CcTx, a first in the κA-family, has been determined by high-resolution NMR spectroscopy together with the analysis of its O-glycan at Ser7. A new type of glycopeptide O-glycan core structure, here registered as core type 9, containing two terminal L-galactose units {α-L-Galp-(1→4)-α-D-GlcpNAc-(1→6)-[α-L-Galp-(1→2)-β-D-Galp-(1→3)-]α-D-GalpNAc-(1→O)}, is highlighted. A sequence comparison to other putative members of the κA-family suggests that O-linked glycosylation might be more common than previously thought. This observation alone underlines the requirement for more careful and in-depth investigations into this type of post-translational modification in conotoxins.

PubMed: 23281027
DOI: 10.1002/CHEM.201202713

実験手法

SOLUTION NMR