4AZ4
E.coli deformylase with Co(II) and hydrosulfide
Summary for 4AZ4
| Entry DOI | 10.2210/pdb4az4/pdb |
| Descriptor | PEPTIDE DEFORMYLASE, COBALT (II) ION, HYDROSULFURIC ACID, ... (4 entities in total) |
| Functional Keywords | hydrolase, cobalt, hydrogen sulfide sensor |
| Biological source | ESCHERICHIA COLI |
| Total number of polymer chains | 1 |
| Total formula weight | 21337.45 |
| Authors | Palm, G.J.,Hinrichs, W. (deposition date: 2012-06-22, release date: 2012-08-22, Last modification date: 2024-10-16) |
| Primary citation | Strianese, M.,Palm, G.J.,Milione, S.,Kuhl, O.,Hinrichs, W.,Pellecchia, C. A Fret Enzyme-Based Probe for Monitoring Hydrogen Sulfide. Inorg.Chem., 51:11220-, 2012 Cited by PubMed Abstract: Fluorescently labeled cobalt peptide deformylase (Co-PDF) can be efficiently used as a fluorescence-resonance-energy-transfer-based sensing device for hydrogen sulfide (H(2)S). The proof of concept of our sensor system is substantiated by spectroscopic, structural, and theoretical results. Monohydrogen sulfide coordination to Co-PDF and Ni-PDF was verified by X-ray crystallography. Density functional theory calculations were performed to gain insight into the characteristics of the coordination adduct between H(2)S and the cobalt cofactor in Co-PDF. PubMed: 23072298DOI: 10.1021/IC301363D PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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