4AZ4

E.coli deformylase with Co(II) and hydrosulfide

4AZ4 の概要

• エントリーDOI: 10.2210/pdb4az4/pdb
• 分子名称: PEPTIDE DEFORMYLASE, COBALT (II) ION, HYDROSULFURIC ACID, ... (4 entities in total)
• 機能のキーワード: hydrolase, cobalt, hydrogen sulfide sensor
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21337.45

構造登録者

Palm, G.J.,Hinrichs, W. (登録日: 2012-06-22, 公開日: 2012-08-22, 最終更新日: 2024-10-16)

主引用文献

Strianese, M.,Palm, G.J.,Milione, S.,Kuhl, O.,Hinrichs, W.,Pellecchia, C.
A Fret Enzyme-Based Probe for Monitoring Hydrogen Sulfide.
Inorg.Chem., 51:11220-, 2012

PubMed Abstract: Fluorescently labeled cobalt peptide deformylase (Co-PDF) can be efficiently used as a fluorescence-resonance-energy-transfer-based sensing device for hydrogen sulfide (H(2)S). The proof of concept of our sensor system is substantiated by spectroscopic, structural, and theoretical results. Monohydrogen sulfide coordination to Co-PDF and Ni-PDF was verified by X-ray crystallography. Density functional theory calculations were performed to gain insight into the characteristics of the coordination adduct between H(2)S and the cobalt cofactor in Co-PDF.

PubMed: 23072298
DOI: 10.1021/IC301363D

実験手法

X-RAY DIFFRACTION (1.8 Å)